青霉素菌渣堆肥中β-内酰胺酶基因丰度变化

发布时间：2018-03-12 12:28

本文选题：青霉素菌渣　切入点：高温堆肥　出处：《中国环境科学》2017年10期 　论文类型：期刊论文

【摘要】：为了解抗生素菌渣堆肥中抗生素残留对抗生素抗性基因(ARGs)环境行为的影响,以青霉素菌渣堆肥为对象,采用实时定量PCR方法分析了8种典型β-内酰胺酶基因,bla_(-TEM)、bla_(-CTX-M-1)、bla_(-CTX-M-9)、bla_(-IMP-1)、bla_(-VIM-2)、bla_(-CMY)、bla_(-OXA-23)、bla_(-NDM-1)在整个堆肥过程中的丰度变化.结果表明,高温堆肥处理大大缩短了青霉素的降解时间;bla-NDM-1在所有样品中均未检出.通过比较β-内酰胺酶基因在不同处理中第1d和30d的绝对数量变化,在处理组中除bla_(-IMP-1)、bla_(-VIM-2)基因绝对数量有所增加外;其他基因都明显减少.从相对丰度看,在堆肥前期,青霉素残留对bla_(-TEM)、bla_(-CTX-M-1)、bla_(-CTX-M-9)、bla_(-CMY)、bla_(-OXA-23)、bla_(-VIM-2)基因有一定诱导富集效应.随着堆肥进程及菌渣堆肥中抗生素浓度的降低,到了堆肥末期,各处理组及对照组bla_(-TEM)、bla_(-CTX-M-1)、bla_(-CTX-M-9)、bla_(-CMY)的相对丰度较堆肥前期显著降低;而处理组中bla_(-IMP-1)、bla_(-VIM-2)的相对丰度较堆肥前期显著增加.
[Abstract]:In order to understand the effect of antibiotic residue on the environmental behavior of antibiotic resistance gene ARGs in antibiotic residue compost, penicillin bacterial residue compost was used as an object. The changes in abundance of eight typical 尾 -lactamase genes, namely, Sertol-CTX-M-1 and Serapor-CTX-M-9, have been analyzed by real-time quantitative PCR. The results show that the variation of the abundance of these eight typical 尾 -lactamase genes in the whole composting process is different from that in the whole composting process. The results show that the variation of the abundance in the whole composting process of the eight typical 尾 -lactamase genes, namely, -CTX-M-1 and -CTX-M-9, has changed in the whole composting process, and the results show that the variation of the abundance in the whole composting process is as follows:. The degradation time of penicillin was shortened greatly by high temperature composting. The absolute quantity of 尾 -lactamase gene in different treatments was compared by comparing the absolute quantity of 尾 -lactamase gene in all samples. In the treatment group, except for the absolute increase in the number of the bla-IMP-1 / VIM-2) gene, the number of other genes decreased significantly in the early stage of composting. Penicillin residue has a certain inductive enrichment effect on the Servo-CTX-M-1 (Servo-CTX-M-9) gene. With the composting process and the concentration of antibiotics in the compost decreasing, the relative abundance of bla-CTX-M-1hkblatibla-CTX-M-1 / CTX-M-9blablablabla-CMY2 is significantly lower than that of the earlier stage of composting. The results show that the effect of penicillin residues on the growth rate of the compost is higher than that of the control group. (2) the relative abundance of CTX-M-9 blablabla-m1 / CTX-M-1 / CTX-M-9 / CMYS is significantly lower than that of the former stage of composting. (2) the relative abundance of CTX-CTX-9 / CMYC is significantly lower than that of the control group (P & amp; lt; 0.05), with the decrease of antibiotic concentration in composting process and in the compost. The relative abundance of blatide-IMP-1 in the treatment group was significantly higher than that in the early stage of composting.
【作者单位】：东南大学能源与环境学院;环境保护部南京环境科学研究所;
【基金】：国家自然青年科学基金资助项目(41401363,41301278) 江苏省自然青年基金资助项目(BK20130102) 环境保护部环保公益性行业科研专项(201209024) 2016年公益性科研院所基本科研业务费项目
【分类号】：S141.4

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