原始红松林退化演替后土壤微生物功能多样性的变化

发布时间：2018-03-12 14:00

本文选题：Biolog-ECO　切入点：土壤微生物　出处：《环境科学研究》2017年06期 　论文类型：期刊论文

【摘要】：为了全面了解原始红松林退化演替为次生林后土壤生境及土壤微生物功能多样性的变化规律,以小兴安岭典型的原始阔叶红松林、退化演替后先锋阶段的白桦林及亚顶级阶段的硬阔叶林为研究对象,采用Biolog-ECO微平板检测法,分析三者0~10 cm和10~20 cm表层土的土壤微生物功能多样性变化规律.结果表明:各林型土壤微生物的AWCD值(平均颜色变化率)随培养时间的延长而增加,培养初期表现为原始阔叶红松林硬阔叶林白桦林;培养末期表现为原始阔叶红松林白桦林硬阔叶林,三者的AWCD值在0~10 cm土层分别为1.06、0.86、0.81,10~20 cm土层分别为0.68、0.47、0.45,原始林显著高于次生林(P0.05),说明原始林土壤微生物对单一碳源的代谢活性显著高于次生林;同一林型下土壤微生物的AWCD值均表现为0~10 cm土层显著高于10~20 cm土层(P0.05).Shannon-Wiener多样性指数、Simpson指数、Mc Intosh指数和Richness丰富度指数也均表现为原始林显著高于次生林(P0.05).原始林土壤微生物对各类碳源的综合利用强度均大于次生林,不同林型下土壤微生物群落的优势碳源类型存在一定的差异,碳水类、氨基酸类、羧酸类和多聚物类碳源是原始林退化演替后土壤微生物群落在碳源利用上发生变化的敏感碳源.
[Abstract]:In order to fully understand the changes of soil habitat and soil microbial functional diversity after the degraded succession of Pinus koraiensis forest into secondary forest, the typical primitive broad-leaved Korean pine forest in Xiaoxingan Mountains was used. The white birch forest in the vanguard stage and the hard broadleaved forest in the subtop stage after the degradation succession were studied. The Biolog-ECO microplate detection method was used. The changes of soil microbial functional diversity in the topsoil of 0 ~ 10 cm and 10 ~ 20 cm were analyzed. The results showed that the AWCD value (average color change rate) of each forest soil microorganism increased with the increase of culture time. In the early stage of culture, the hard broad-leaved birch forest was found in the original broad-leaved Korean pine forest, and at the end of the culture period, the hard-leaved forest in the white birch forest of the primitive broad-leaved Korean pine forest. The AWCD values of the three species in the 0 ~ (10 cm) soil layer were 1 06 ~ 0. 86 ~ 0. 86 ~ 0. 81 ~ 0. 01 ~ 10 ~ (20 cm) soil layer, respectively, and 0. 68 ~ 0. 47 ~ 0. 57 ~ 0. 45 cm soil layer, which indicated that the metabolic activity of soil microorganism to a single carbon source in the primary forest was significantly higher than that in a secondary forest. The AWCD value of soil microorganism in the same forest type was significantly higher than that in 10 ~ 20 cm soil layer (P 0.05N. Shannon-Wiener diversity index) Mc Intosh index and Richness richness index. It was also shown that the AWCD value of soil microorganism in the primary forest was significantly higher than that in the secondary forest (P 0.05), and that in the primitive forest soil was significantly higher than that in the primary forest. The comprehensive utilization intensity of various carbon sources by microorganisms was higher than that of secondary forest. There are some differences in the dominant carbon source types of soil microbial communities under different forest types, such as carbohydrates, amino acids, carbohydrates and amino acids. Carboxylic acid and polymeric carbon sources are sensitive carbon sources for the change of soil microbial community in carbon source utilization after the degradation of primitive forest.
【作者单位】：东北林业大学生命学院;黑龙江省科学院自然与生态研究所湿地与生态保育国家地方联合工程实验室;
【基金】：国家自然科学基金项目(31670496) 中央高校基本科研业务费专项(2572015EA02)
【分类号】：S714.3

【相似文献】