不同施肥模式和地下水位条件下红壤水稻土PLFA指纹特征

发布时间：2018-03-14 21:01

本文选题：长期施肥　切入点：地下水位　出处：《生态与农村环境学报》2017年06期 　论文类型：期刊论文

【摘要】：土壤微生物群落是评估土壤质量、衡量土壤肥力和指示土壤环境变化的重要指标。施肥和地下水位均会引起土壤养分和微环境发生变化,但地下水位对土壤微生物群落影响的研究尚鲜有报道,特别是施肥和地下水位管理双因素协同作用对土壤微生物群落的影响还不清楚。采用磷脂脂肪酸(PLFA)指纹图谱法研究长期施肥和不同地下水位条件下红壤水稻土微生物群落。结果表明,不同地下水位管理显著影响土壤pH值、E_(h,p)(排水状态下的氧化还原电位)和NO_3~--N含量,不同施肥模式显著改变土壤Eh,y(淹水状态下的氧化还原电位)、E_(h,p)、有机碳含量、全氮含量和速效钾含量。不同地下水位显著影响土壤总PLFA、细菌PLFA、G+PLFA含量及G+PLFA/GPLFA比值,如高地下水位土壤G+PLFA/G-PLFA比值(0.40~0.51)明显低于低地下水位土壤(0.40~1.94);不同施肥模式显著影响G-PLFA含量和G+PLFA/G-PLFA比值,如施有机肥土壤G+PLFA/G-PLFA比值(0.40~1.94)明显高于施化肥土壤(0.40~0.46)。逐步回归分析显示放线菌PLFA含量显著受土壤pH值及全氮含量的影响,G+、厌氧菌、好氧菌PLFA含量显著受土壤pH值的影响,真菌PLFA/细菌PLFA比值显著受土壤Eh,y的影响,G+PLFA/G-PLFA比值显著受NO3--N含量和全钾含量的影响。主成分分析显示环境因子影响了长期施肥和不同地下水位条件下红壤水稻土微生物群落结构,全钾含量、pH值和Eh,y是导致LNOM处理与其他处理间微生物群落结构不同的主要环境因子,而NO3--N含量、全氮含量、全磷含量是导致LHOM处理与HNOM、HHOM、LCF、HCF处理间微生物群落结构不同的主要环境因子。可见,长期施肥和地下水位管理显著影响红壤水稻土理化性质及微生物群落。
[Abstract]:Soil microbial communities are important indicators for evaluating soil quality, measuring soil fertility and indicating changes in soil environment. Both fertilization and groundwater level can cause changes in soil nutrients and microenvironments. However, there are few reports on the effect of groundwater level on soil microbial community. Especially, the synergistic effect of fertilization and groundwater level management on soil microbial community is not clear. Study on microflora of paddy soil in red soil by using PLFA-PLFA-fingerprint method under long-term fertilization and different groundwater level conditions. Biota. The results show that. Different groundwater level management significantly affected soil pH value (redox potential under drainage state) and NO_3~--N content, and different fertilization patterns significantly changed soil Ehnyr (redox potential under flooded condition), organic carbon content. Total nitrogen content and available potassium content. Different groundwater levels significantly affected soil total PLFAA, bacterial PLFAG PLFA content and GPLFA / GPLFA ratio. For example, the G PLFA/G-PLFA ratio of high groundwater level soil was significantly lower than that of low groundwater level soil, and different fertilization patterns significantly affected G-PLFA content and G PLFA/G-PLFA ratio. For example, G PLFA/G-PLFA ratio of soil applying organic fertilizer was significantly higher than that of chemical fertilizer soil. Stepwise regression analysis showed that PLFA content of actinomycetes was significantly affected by soil pH value and total nitrogen content. PLFA content of anaerobic bacteria and aerobic bacteria was significantly affected by soil pH value. The PLFA ratio of fungal PLFA/ bacteria was significantly affected by the content of NO3--N and total potassium. Principal component analysis showed that environmental factors affected the microbial community structure of paddy soil in red soil under long-term fertilization and different groundwater levels. Total potassium content, pH value and Ehlany were the main environmental factors leading to the difference of microbial community structure between LNOM treatment and other treatments, while NO3--N content and total nitrogen content, Total phosphorus content was the main environmental factor leading to the difference of microbial community structure between LHOM treatment and HNOMN HHOMM-LCFHCF treatment. It can be seen that long-term fertilization and groundwater level management significantly affected the physical and chemical properties and microbial community of paddy soil in red soil.
【作者单位】：江苏省物质循环与污染控制重点实验室/南京师范大学地理科学学院;南京科技职业学院;南京师范大学环境学院;湖南农业大学资源环境学院;
【基金】：国家自然科学基金(41401293,41271255) 江苏省自然科学基金(BK20141051)
【分类号】：S154.3

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