不同生长模式下人参土壤微生物群落结构研究

发布时间：2018-03-15 03:17

本文选题：人参　切入点：微生物群落结构　出处：《东北农业大学学报》2016年02期 　论文类型：期刊论文

【摘要】：为明确不同生长模式下人参土壤微生物群落结构组成,以野山参、林下参和农田栽参土壤作为试验材料,利用磷脂脂肪酸分析法(PLFA)测定人参土壤微生物群落结构组成。结果表明,不同生长模式下人参土壤中C、N含量不同,野山参土壤中全C和全N含量均显著高于林下参和农田栽参土壤(P0.05),是农田栽参土壤的10.30和4.61倍;C/N比为野山参土壤林下参土壤农田栽参土壤,野山参和林下参土壤C/N比差异不显著,但远高于农田栽参土壤;野山参土壤微生物总量最高为1 671.05 nmol·g~(-1),农田栽参土壤最低为266.80 nmol·g~(-1)。不同人参生长模式下细菌均为优势菌群,但放线菌在野山参土壤中含量为187.18 nmol·g~(-1),林下参土壤中为26.12nmol·g~(-1),农田栽参土壤中为6.77 nmol·g~(-1)。通过挖掘功能微生物发现,代表抗逆性微生物指标蓝细菌(182ω6)及革兰氏阳性菌与革兰氏阴性菌比值(G+/G-)均为野山参土壤林下参土壤农田栽参土壤,野山参土壤中蓝细菌含量是林下参土壤的3.51倍,农田栽参土壤则未检出;野山参土壤中G+/G-分别是林下参土壤和农田栽参土壤的6.93和9.10倍。野山参土壤微生物量大、种群丰度高,生产实践中可通过添加外援微生物改变土壤微生物群落结构及组成提高农田栽参土壤肥力。
[Abstract]:In order to determine the structure of soil microbial community of Panax ginseng under different growth patterns, the soil of Panax ginseng, undergrowth ginseng and cultivated ginseng were used as experimental materials. The microbial community composition of ginseng soil was determined by phospholipid fatty acid analysis (PLFAA). The results showed that the contents of Con N in ginseng soil were different under different growth patterns. The contents of total C and N in the soil of Panax ginseng were significantly higher than those in the soil of Panax ginseng and cultivated ginseng, which was 10.30 and 4.61 times of that in the soil of cultivated ginseng, and the ratio of C / N to C / N was 10.30 and 4.61 times higher than that in the soil of Panax ginseng and the ratio of C / N to C / N in the soil. There was no significant difference in C / N ratio between wild ginseng and undergrowth ginseng soil, but it was much higher than that in farmland soil, the total microbial amount of wild mountain cucumber soil was 1 671.05 nmol 路g ~ (-1) ~ (-1), and the lowest was 266.80 nmol 路g ~ (-1) ~ 1 ~ (-1). Bacteria were dominant flora under different growth patterns of Panax ginseng. However, the content of actinomycetes in the soil of wild ginseng was 187.18 nmol 路GG ~ (-1), that in the soil of ginseng was 26.12 nmol 路g ~ (-1) ~ (-1), and that in cultivated soil was 6.77 nmol 路g ~ (-1) 路g ~ (-1). The results were as follows: (1) by excavating functional microorganism, the content of actinomycetes in the soil was 187.18 nmol. The blue bacteria (182 蠅 6) and the ratio of Gram-positive bacteria to Gram-negative bacteria (G / G-G), which represent resistance to stress, were all found to be 3.51 times higher than those in the soil of Radix pilosula, which were planted in farmland, and the content of cyanobacteria in the soil was 3.51 times higher than that in the soil of Radix Panax ginseng. The G / G- in the soil was 6.93 and 9.10 times higher than that in the undergrowth soil and the cultivated soil, respectively. The soil microbial biomass was large and the population abundance was high, the results showed that the G / G- ratio in the soil was 6.93 and 9.10 times higher than that in the undergrowth soil and the cultivated soil, respectively. The structure and composition of soil microbial community can be changed by adding external microorganism in the production practice, and the soil fertility of cultivated ginseng can be improved.
【作者单位】：中国农业科学院特产研究所;
【基金】：吉林省科技厅项目(20150204053NY)
【分类号】：S567.51;S154.3

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