四种Bt蛋白对六种重要鳞翅目害虫的杀虫活性评价

发布时间：2018-03-16 05:22

本文选题：Bt　切入点：鳞翅目害虫　出处：《中国生物防治学报》2017年06期 　论文类型：期刊论文

【摘要】：筛选对靶标害虫高效、低毒的Bt蛋白对未来开发新型Bt生物杀虫剂、转基因抗虫植物新品种等具有重要意义。我们在室内测定了Cry2Ab4、Cry2Ah1、Cry1Ca7及Vip3Aa11四种Bt蛋白对棉铃虫、甜菜夜蛾、斜纹夜蛾、黏虫、玉米螟、二化螟的杀虫效果。结果表明,Cry2Ab4杀虫谱广、对六种鳞翅目的重要害虫都具有很高的杀虫效果,尤其是对棉铃虫、甜菜夜蛾、斜纹夜蛾和玉米螟的毒力效果显著;Cry2Ah1对斜纹夜蛾、甜菜夜蛾具有很好的致死作用,对棉铃虫、玉米螟和二化螟也具有较好的杀虫效果,但对黏虫只有明显的体重抑制作用、致死效果不明显;Cry1Ca7对甜菜夜蛾和斜纹夜蛾有明显的防治效果,其次对棉铃虫、二化螟也有较好的杀虫作用,但对玉米螟毒力效果较差,对黏虫没有毒力作用;Vip3Aa11对甜菜夜蛾和斜纹夜蛾的防治效果好,对玉米螟、棉铃虫和二化螟有较好的防治效果,但对黏虫没有毒力作用。
[Abstract]:Screening BT proteins with low toxicity and high efficiency to target insect pests is of great significance for the development of new BT biological insecticides and transgenic insect-resistant plant varieties. Four BT proteins, Cry2Ab4, Cry2Ah1, Cry1Ca7 and Vip3Aa11, were tested in laboratory against cotton bollworm and Spodoptera exigua. The results showed that Cry2Ab4 had a wide range of insecticidal effects on six important pests of Lepidoptera, especially cotton bollworm and Spodoptera exigua. The virulence of Spodoptera litura and Corn Borer was remarkable. Cry2Ah1 had a good lethal effect on Spodoptera litura and Spodoptera exigua, and had good insecticidal effect on cotton bollworm, corn borer and stem borer. The lethal effect of Cry1Ca7 on Spodoptera exigua and Spodoptera litura was not obvious, followed by Helicoverpa armigera and Chilo suppressalis, but the toxicity of Cry1Ca7 to corn borer was poor. Vip3Aa11 had no virulence effect on Spodoptera exigua and Spodoptera litura, and had better control effect on corn borer, cotton bollworm and stem borer, but had no virulence effect on slime worm.
【作者单位】：中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室;
【基金】：国家转基因生物新品种培育重大专项(2016ZX08011-002)
【分类号】：S433.4

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