两种不同化学农药抗性背景小菜蛾对BtCry1Ac蛋白敏感度的比较

发布时间：2018-03-18 05:35

本文选题：小菜蛾　切入点：化学农药　出处：《中国生物防治学报》2017年06期 　论文类型：期刊论文

【摘要】：为在小菜蛾防治中合理使用化学杀虫剂和生物农药Bt,探究了小菜蛾对化学农药的抗药性与其对Bt杀虫蛋白Cry1Ac的敏感度之间的关系。采用浸叶法测定了2个抗性背景不同的小菜蛾种群对9种常用杀虫剂和Cry1Ac蛋白的敏感度,通过比较敏感度差异,初步探索了小菜蛾对化学农药的抗药性和其对Cry1Ac蛋白敏感度之间的关系。结果表明:采自广东博罗县田间的小菜蛾种群(GDBL)对除Cry1Ac之外的9种常用杀虫剂,均已产生了不同程度的抗药性;而北京室内小菜蛾种群(BJNK),虽然对高效氯氰菊酯、阿维菌素、茚虫威、多杀菌素、溴虫腈具有抗性,对Cry1Ac在内的其他5种杀虫剂仍比较敏感。可见,对多种不同类型的杀虫剂产生了不同程度抗性的两个小菜蛾种群,对Cry1Ac蛋白仍处于敏感状态。由于小菜蛾对化学杀虫剂的抗性机制与其对Cry1Ac的抗性机制不同,小菜蛾对化学杀虫剂的抗药性与其对Cry1Ac的抗药性之间不存在交互抗性。因此,在田间可以通过加强Bt杀虫剂的使用来提高对多种化学杀虫剂产生抗性的小菜蛾的防治效果。
[Abstract]:In order to reasonably use chemical insecticides and biological pesticides BT in controlling Plutella xylostella, the relationship between resistance of diamondback moth to chemical pesticides and sensitivity to BT insecticidal protein Cry1Ac was studied. Sensitivity of different diamondback moth populations to 9 common insecticides and Cry1Ac proteins, The relationship between the resistance of Plutella xylostella to chemical pesticides and the sensitivity of diamondback moth to Cry1Ac protein was studied by comparing the sensitivity. The results showed that the population of Plutella xylostella collected from the field of Boro County, Guangdong Province, was resistant to 9 kinds of insecticides except Cry1Ac. BJNKK, although resistant to cypermethrin, abamectin, indene, spinosin, brominonitrile, and cypermethrin. It is still sensitive to five other insecticides, including Cry1Ac. It can be seen that there are two populations of diamondback moths with different resistance to many different types of insecticides. Because the resistance mechanism of diamondback moth to chemical insecticide is different from that of Cry1Ac resistance, there is no cross resistance between diamondback moth resistance to chemical insecticide and its resistance to Cry1Ac. The control effect of diamondback moth resistant to many kinds of chemical insecticides can be improved by strengthening the use of BT insecticides in the field.
【作者单位】：中国农业大学植物保护学院;中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室;山西师范大学;广东省农业科学院植物保护研究所;
【基金】：国家重点研发计划(2017YFD0201204)
【分类号】：S433.4

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