小麦根际产ACC脱氨酶细菌种群的多样性研究

发布时间：2018-03-19 19:44

本文选题：ACC脱氨酶　切入点：根际细菌　出处：《西北农林科技大学学报(自然科学版)》2017年06期 　论文类型：期刊论文

【摘要】：【目的】对杨凌和长武两地小麦根际土壤中能产生1-氨基环丙烷-1-羧酸(ACC)细菌的多样性进行调查比较。【方法】以ACC为唯一氮源从小麦根际土壤中富集和纯化产ACC脱氨酶细菌,使用Mi-seq高通量测序平台对富集产物进行多样性检测,构建16SrDNA基因克隆文库,对所分离的产ACC脱氨酶细菌进行系统发育分析;利用R语言绘图,对两地区该类细菌群落结构进行对比研究。【结果】长武地区小麦根际土壤2个样品中优化后的序列共计55 715条,基于97%的相似性被划分为35个OTU,Shannon多样性指数为1.43和0.6,Simpson多样性指数为0.380 5和0.773 7,经系统发育分析,主要隶属于假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacteriaceae)、无色杆菌属(Achromobacter)、不动杆菌属(Acinetobacter)和乳球菌属(Lactococcus),所占比例分别为73.26%,15.86%,4.03%,3.26%和1.87%。杨凌地区小麦根际土壤2个样品中优化后序列共计34 044条,基于97%的相似性被划分为37个OTU,Shannon多样性指数为2.21和2.38,Simpson多样性指数为0.181 7和0.140 4,经系统发育分析,主要隶属于肠杆菌属(Enterobacteriaceae)、无色杆菌属(Achromobacter)、假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、寡养单胞菌属(Stenotrophomonas),所占比例分别为37.66%,23.51%,14.90%,9.07%和5.80%。【结论】杨凌、长武地区小麦产ACC脱氨酶根际促生细菌的种类有部分重叠,但丰度差异较大,在空间分布上呈现一定程度的地缘隔离,调查结果可用于指导产ACC脱氨酶根际促生菌的资源开发及施用效果研究。
[Abstract]:[objective] to investigate and compare the diversity of 1-aminocyclopropane-1-carboxylic acid (ACC) producing bacteria in wheat rhizosphere soil between Yang Ling and Changwu. [methods] ACC deaminase producing bacteria were enriched and purified from wheat rhizosphere soil with ACC as the sole nitrogen source. Using Mi-seq high-throughput sequencing platform to detect the diversity of enriched products, construct 16s rDNA gene clone library, analyze the phylogeny of the isolated ACC deaminase bacteria. The community structure of this kind of bacteria was compared between the two regions. [results] 55,715 optimized sequences were obtained from two samples of wheat rhizosphere soil in Changwu area. The similarity based on 97% was divided into 35 OTU Shannon diversity indices of 1.43 and 0.6 Simpson diversity indices of 0.380 5 and 0.773 7, respectively. Phylogenetic analysis was performed. It mainly belongs to Pseudomonas, Enterobacteriaceaeae, Achromobacter, Acinetobacter, Acinetobacter and Lactococcus, the proportions of which are 73.260.864.033.26% and 1.877.The optimized sequence in two samples of wheat rhizosphere soil in Yang Ling area is 34 044. The similarity based on 97% was divided into 37 OTU Shannon diversity indices of 2.21 and 2.38 Simpson diversity indices of 0.1817 and 0.1404, respectively. It is mainly belong to Enterobacteriaceaeae, chromobacter, Pseudomonas, Bacillus, Stenotrophomonas oligotrophoniae, and the proportions are 37.66% and 5.80%, respectively. [conclusion] Yang Ling has some overlapping species of ACC deaminase promoting rhizosphere bacteria in Changwu area. But the abundance is quite different, showing a certain degree of geographical isolation in the spatial distribution, the results of the investigation can be used to guide the development of ACC deaminase producing rhizosphere promoting bacteria resources and application effect.
【作者单位】：西北农林科技大学生命科学学院;
【基金】：国家“863”计划项目(2011AA100504)
【分类号】：S154.381

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