毕赤酵母G5拮抗葡萄灰霉病机理初探

发布时间：2018-03-20 05:18

  本文选题：葡萄灰霉病　切入点：生物防治　出处：《生物技术通报》2017年09期 　论文类型：期刊论文

【摘要】：针对新疆红提葡萄中分离的生防菌株——毕赤酵母G5,以葡萄灰霉菌(Botrytis cinerea)为靶标菌,研究了G5对葡萄灰霉菌孢子及对葡萄果实内5种关键酶活性的影响,初步探讨了G5拮抗葡萄采后灰霉病的机理。采用离体(in vitro)实验与活体(in vivo)实验的方法对毕赤酵母菌G5拮抗葡萄灰霉病的机理进行探究。结果发现,毕赤酵母菌株G5对葡萄灰霉菌孢子的萌发和菌丝生长均有抑制作用,对灰霉菌丝的抑制率最高为80.20%,对灰霉孢子抑制率达86.89%;两者间存在营养竞争关系,有重寄生现象产生,拮抗菌自身并不分泌抗菌物质。此外,该菌株能诱导果实体内过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶、几丁质酶、β-1,3-葡聚糖酶的酶活性,使其酶活性有显著提高,说明毕赤酵母G5可以有效的诱导果实内抗病相关酶的活性,增强对灰霉病的抑制效果。毕赤酵母G5拮抗葡萄灰霉病的机理主要包括营养竞争、诱导抗病性,是否有重寄生作用还需要进一步验证。
[Abstract]:The effects of G5 on the spores of Grapevine Ash and the activities of five key enzymes in grape fruit were studied by using Botrytis cinerea, a strain of Pichia pastoris G5, isolated from red extract grape in Xinjiang as the target strain. The mechanism of antagonistic effect of G5 on gray mold of grape was studied. The mechanism of antagonistic effect of Pichia pastoris G5 on gray mold of grape was studied by in vitro and in vivo vivoo experiments. Pichia pastoris strain G5 inhibited the spore germination and hyphal growth of Aspergillus grapevine, the highest inhibition rate was 80.20%, and the inhibition rate against Aspergillus grapevine spores was 86.89%. There was a nutritional competition between the two strains, and there was a phenomenon of heavy parasitism. In addition, the antagonistic bacteria could induce the activities of peroxidase, polyphenoloxidase, phenylalanine ammonia-lyase, chitinase, 尾 -1C 3-glucanase in the fruit, and the enzyme activity was significantly increased. The results showed that Pichia pastoris G5 could effectively induce the activity of disease-related enzymes in the fruit and enhance the inhibition effect on gray mold. The mechanism of antagonistic effect of Pichia pastoris G5 against gray mold of grape mainly included nutrition competition and induced disease resistance. The existence of hyperparasitism needs further verification.
【作者单位】： 石河子大学食品学院;
【基金】：兵团博士资金专项(2014BB006)
【分类号】：S436.631;S476.1
，

本文编号：1637687