两株解磷细菌的解磷活性及作用机制研究

发布时间：2018-03-20 14:44

本文选题：解磷细菌　切入点：解磷活性　出处：《中国生态农业学报》2017年03期 　论文类型：期刊论文

【摘要】：解磷细菌在增加土壤可溶性磷含量、提高磷肥利用效率方面具有重要作用。为选筛高效解磷菌、探讨其解磷机制,本文利用平板溶磷圈法筛选解磷细菌,采用钼锑抗比色法研究其解磷活性,苯磷酸二钠法研究其磷酸酶活性,利用薄层层析分析其产生的有机酸,根据生理生化特征和16S r RNA基因序列系统发育分析,确定其分类学地位。结果表明,菌株JXJ-11和JXJ-15对植酸钙的降解活性很强,3 d后培养液中可溶性磷浓度分别增加219 mg·L~(-1)和216 mg·L~(-1);对磷酸钙降解活性较弱,最高可溶性磷浓度仅为植酸钙的21.79%~30.37%;解磷细菌可分泌酸性、中性和碱性磷酸酶,降解不溶性磷,可能产生丙酸和琥珀酸等有机酸,降低培养液p H,增加可溶性磷浓度。两株细菌均为革兰氏阴性杆菌,无芽孢,产生硫化氢,其中菌株JXJ-11的16S rRNA基因序列与Sphingomonas melonis DAPP-PG 224T和S.aquatilis JSS7T相似性最高(99.79%),菌株JXJ-15的16S rRNA基因序列与Klebsiella pneumoniae subsp.pneumoniae DSM 30104T相似性最高(99.73%),根据以上信息,确定菌株JXJ-11和JXJ-15分别是鞘氨醇单胞菌属和克雷白氏杆菌属的成员。菌株JXJ-11和JXJ-15的解磷机制包括分泌有机酸和磷酸酶,其中JXJ-11在微生物磷肥研制方面具有潜在应用价值。
[Abstract]:Phosphorus bacteria play an important role in increasing the content of soluble phosphorus in soil and improving the utilization efficiency of phosphorus fertilizer. The phosphatase activity was studied by molybdenum antimony anti-colorimetric method, the phosphatase activity by benzene disodium phosphate method, the organic acids produced by TLC, and phylogenetic analysis according to physiological and biochemical characteristics and 16s r RNA gene sequence. The taxonomic status was determined. The results showed that the concentration of soluble phosphorus in the culture medium increased 219mg 路L ~ (-1) and 216mg 路L ~ (-1), respectively, but the biodegradation activity of calcium phosphate was weaker than that of the strain JXJ-11 and JXJ-15, after 3 days, the concentration of soluble phosphorus in the culture medium was increased by 219mg 路L ~ (-1) and 216mg 路L ~ (-1), respectively. The highest soluble phosphorus concentration is only 21.79% of calcium phytate. The bacteria can secrete acid, neutral and alkaline phosphatase, degrade insoluble phosphorus, and may produce organic acids such as propionic acid and succinic acid. Both strains were Gram-negative bacilli, spore free, hydrogen sulphide. The 16s rRNA gene sequence of strain JXJ-11 has the highest similarity with Sphingomonas melonis DAPP-PG 224T and S.aquatilis JSS7T. The 16s rRNA gene sequence of JXJ-15 has the highest similarity with Klebsiella pneumoniae subsp.pneumoniae DSM 30104T. According to the above information, the 16s rRNA gene sequence of strain JXJ-15 has the highest similarity with Klebsiella pneumoniae subsp.pneumoniae DSM 30104T. Strains JXJ-11 and JXJ-15 were identified as members of sphingomonas and Klebsiella, respectively. The dephosphorization mechanisms of JXJ-11 and JXJ-15 included the secretion of organic acids and phosphatase, among which JXJ-11 had potential application value in the development of microbial phosphate fertilizer.
【作者单位】：九江学院药学与生命科学学院;
【基金】：江西省农业科技支撑计划项目(20121BBF60048)资助~~
【分类号】：S182

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