自然微生物挂膜处理水产养殖废水的效果及微生物群落分析

发布时间：2018-03-20 20:34

本文选题：生物填料　切入点：PHBV　出处：《水生生物学报》2017年06期 　论文类型：期刊论文

【摘要】：以弹性填料和流化床填料为硝化反应的生物挂膜材料,聚羟基丁酸/戊酸共聚酯(PHBV)为反硝化反应的碳源和生物膜载体,通过微生物自然挂膜处理低C/N比水产养殖废水,去除水体中的氨氮、亚硝酸盐氮及总氮。应用Miseq高通量测序技术对生物膜的微生物群落组成和结构进行分析。结果表明:温度25—30℃,该处理系统首次挂膜成功需要4周,启动后运行稳定,对2种不同来源和氮污染程度的养殖废水均有较好的脱氮效果,氨氮、亚硝酸盐氮及总氮的去除率均在90%以上。硝化生物膜(a)的优势菌分别归属变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)。反硝化生物膜(b)微生物群落的多样性指数和丰度指数均远大于前者,主要为变形菌门、厚壁菌门、拟杆菌门、螺旋体门(Spirochaetae)及绿菌门(Chlorobi)。其中,归属于变形菌门β-变形菌纲(Betaproteobacteria)的丛毛单胞菌科(Comamonadaceae)和红环菌科(Rhodocyclaceae)在2种生物膜中占比均较高。由于所处环境(载体,碳源、溶氧等)不同,在属分类水平上,2种生物膜的细菌群落结构表现出明显差异。生物膜a中属的种类仅为b的三分之二,相对丰度0.5%的优势菌属,a为8个,b为18个。其中,隶属丛毛单胞菌科和红环菌科未知属的优势种群分别占到a、b总序列数的56.67%和45.51%。磁螺菌属(Magnetospirillum)和硝化螺菌属(Nitrospira)是a中特有的优势功能菌群,梭菌属(Clostridium)、动胶菌属(Zoogloea)、管道杆菌属(Cloacibacterium)、脱硫弧菌属(Desulfovibrio)等具有反硝化功能的菌群为b的优势菌属。
[Abstract]:Using elastic filler and fluidized bed packing as biofilm material for nitrification and polyhydroxybutyric acid / valerate copolyester (PHBV) as carbon source and biofilm carrier for denitrification treatment of low C / N ratio aquaculture wastewater was studied. Ammonia nitrogen, nitrite nitrogen and total nitrogen were removed from the water. The microbial community composition and structure of biofilm were analyzed by Miseq high-throughput sequencing technique. After start-up, the operation is stable, and it has better denitrification effect on two kinds of aquaculture wastewater from different sources and degree of nitrogen pollution, ammonia nitrogen, ammonia nitrogen, ammonia nitrogen, ammonia nitrogen, ammonia nitrogen, ammonia nitrogen, The removal rates of nitrite nitrogen and total nitrogen are above 90%. The dominant bacteria of nitrifying biofilm belong to the diversity index and abundance index of Proteobacteriae (Proteobacteriae), Bacteroidetes (Bacteroidetes.) and Firmicutes.denitrifying biofilm (b). The number is much larger than the former. It mainly consists of phylum, phylum, Bacteroides, Spirochaetaees, Spirochaetaees and Chlorobiridae. Comamonadaceaees and Rhodocyclaceaes belonging to Beta proteobacteriae, belong to the class 尾 -Proteobacteriae, have a higher proportion in the two biofilms. Because of the different environment (carrier, carbon source, dissolved oxygen, etc.), At the taxonomic level, the community structure of the two biofilms showed obvious differences. The species of biofilm a were only 2/3 of b, and the dominant genera of relative abundance 0.5% were 8 nb and 18, respectively. 56.67% and 45.51of the total number of AGB sequences were obtained from the dominant populations belonging to the unknown genera of Cytomonas and Rhodoidae, respectively. Magnetospirillum (Magnetospirillum) and Nitrospira (Nitrospiraa) were the dominant functional flora of A. Clostridium, Zoogloea, Cloacia, Desulfovibrio and other bacteria with denitrification are dominant genera.
【作者单位】：浙江省淡水水产研究所;
【基金】：“十二五”农村领域国家科技计划课题(2015BAD13B04) 湖州市生态文明先行示范区科技专项重点项目(2014ZD2020)资助~~
【分类号】：X172;X714

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