不同土壤灭菌方法对土壤微生物活性的影响

发布时间：2018-03-25 02:14

本文选题：土壤灭菌　切入点：土壤微生物活性　出处：《复旦学报(自然科学版)》2017年06期

【摘要】：灭菌处理是土壤生态学、微生物学及其相关学科的一种重要研究手段,灭菌效果的好坏直接关系到后续研究的进行及其结果的准确性.常用土壤灭菌方法的灭菌效果及其评估仍然存在很大的不确定性.本文选取目前常用的3种灭菌方法,即高温高压蒸汽法、氯仿熏蒸法及辐射灭菌法,对不同背景的土壤样品进行灭菌.通过改变灭菌次数及灭菌间隔时间,观察灭菌后土壤微生物呼吸速率的变化.结果表明:(1)常规氯仿熏蒸灭菌后土壤微生物呼吸速率并不完全随着微生物量的减小而降低,说明单纯从土壤微生物量的角度不能准确界定灭菌效果以及解析微生物在土壤生态过程中的作用;(2)高温高压蒸汽比常规氯仿熏蒸和辐射灭菌方法有更好的土壤微生物灭活效果.实际应用中采用高温高压蒸汽灭菌时,建议采用灭菌2次、时间间隔为3d的灭菌处理;在相关研究中,对使用该方法灭菌效果的分析研究最佳时间不宜超过2d.
[Abstract]:Sterilization of soil ecology, an important research means of Microbiology and related disciplines, the accuracy of sterilization effect is directly related to the follow-up research and its results. The sterilization effect and is used to evaluate the soil sterilization method still has great uncertainty. This paper selects 3 kinds of high temperature and high pressure steam sterilization method, i.e. method, chloroform fumigation and radiation sterilization, sterilization of soil samples with different background. By changing the number of sterilization and sterilization time interval, the change of soil microbial respiration rate after sterilization was observed. The results showed that (1) the conventional CHLOROFORM FUMIGATION after sterilization of soil microbial respiration rate is not entirely with the decrease of microbial biomass decreased. A simple description from the angle of soil microbial biomass can not accurately define the sterilization effect and in soil ecological processes for the analysis of microbial; (2) High temperature and high pressure steam have better soil microbial inactivation effect than conventional CHLOROFORM FUMIGATION and radiation sterilization method. High temperature and high pressure steam sterilization in the practical application, recommend the use of sterilized 2 times, the time interval for sterilization of 3D; in related studies, analysis of the best time of not using the method of sterilization effect of more than 2D.

【作者单位】：复旦大学生命科学学院生物多样性科学研究所;
【基金】：国家自然科学基金(31670491) 上海浦江学者计划(16PJ1400900)
【分类号】：S154.3

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