溶磷菌对大豆根际土壤酶活性及微生物群落的影响

发布时间：2018-04-03 04:34

本文选题：溶磷菌　切入点：盐碱土改良　出处：《中国农业大学学报》2017年11期

【摘要】：为研究添加外源溶磷菌wj1和wj3对大豆根际土壤的影响及其改良盐碱土的可行性,以吉林省主推高产高油大豆品种"吉育406"为材料,将wj1和wj3菌液施入到不同处理的土壤中,分析外源溶磷菌对不同生育时期大豆根际土壤酶活性、土壤微生物数量和微生物群落的变化。结果表明:大豆生长的R2和R6两时期,混合菌能显著增加黑土中磷酸酶、脲酶、蔗糖酶和蛋白酶的活性;菌株wj1在整个生育期内提高了黑土磷酸酶活性;黑土添加溶磷菌对大豆根际各种微生物数量均有提高;菌株wj1有利于促进黑土中溶磷菌和自身固氮菌数量的增加,添加wj3和混合菌对整个生育期内土壤中氨化细菌和硝化细菌的数量增加效果明显;添加溶磷菌可显著增加混合盐碱土壤中微生物数量,改善土壤磷酸酶、脲酶和蔗糖酶活性;加菌处理的土壤微生物群落多样性与未接菌处理差异明显;V3-R6,黑土与混合土壤加菌处理均提高了土壤Shannon指数与微生物群落丰富度,增强了混合盐碱土中微生物代谢能力。添加外源溶磷菌可有效增加黑土及混合盐碱土土壤微生物数量,使土壤营养水平趋于合理。
[Abstract]:In order to study the effect of exogenous phosphorus soluble bacteria wj1 and wj3 on soybean rhizosphere soil and the feasibility of improving saline-alkali soil, Jilin Province high yield and high oil soybean variety "Jiyu 406" was used as the material, wj1 and wj3 bacteria solution were applied to different treatments soil.The changes of enzyme activity, soil microbial quantity and microbial community of soybean rhizosphere at different growth stages were analyzed.The results showed that the activities of phosphatase, urease, sucrase and protease in black soil were significantly increased by mixed bacteria in R2 and R6 stage of soybean growth, and the activity of phosphatase in black soil was increased by strain wj1 during the whole growth period.The addition of phosphorus solubilizing bacteria to black soil increased the number of various microbes in soybean rhizosphere, and the strain wj1 was helpful to increase the number of phosphorus soluble bacteria and nitrogen-fixing bacteria in black soil.The addition of wj3 and mixed bacteria could significantly increase the number of ammoniated bacteria and nitrifying bacteria in soil during the whole growth period, and the addition of phosphorus solubilizing bacteria could significantly increase the number of microbes in mixed saline-alkali soil and improve the activities of phosphatase, urease and sucrase in soil.The diversity of soil microbial community was significantly different from that of non-inoculated soil treated with bacteria. The soil Shannon index and microbial community richness were increased by adding bacteria to black soil and mixed soil, and the ability of microbial metabolism in mixed saline-alkali soil was enhanced.The addition of exogenous phosphorus solubilizing bacteria can effectively increase the number of soil microbes in black soil and mixed saline-alkali soil and make the soil nutrition level more reasonable.
【作者单位】：吉林农业大学农学院;吉林农业大学生命科学院;
【基金】：国家自然科学基金(31201687) 吉林省自然科学基金(20170101160JC)
【分类号】：S154;S565.1

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