微生物多样性对土壤碳代谢特征的影响

发布时间：2018-04-09 01:24

本文选题：微生物多样性　切入点：碳矿化速率　出处：《环境科学》2017年10期

【摘要】：土壤微生物群落在生态系统过程中发挥着重要的作用,而关于土壤微生物多样性对生态系统功能的影响无一致结论.为了研究微生物多样性对土壤碳代谢特征的影响,将稀释10~(-1)、10~(-3)和10~(-5)倍(D1、D3和D5)的庞泉沟阔叶混交林土壤悬浮液接种在灭菌的土样中.通过碱式滴定法和Biolog Eco板等实验方法测定了不同多样性梯度下土壤碳矿化速率和碳代谢利用模式的变化.结果表明培养6周后,D1处理的碳矿化速率、累积碳矿化量、平均孔颜色变化率(AWCD)和多样性指数(Shannon、Mc Intosh和丰富度指数)均显著高于D5处理.且相关分析表明,累积碳矿化量和AWCD与丰富度呈显著负相关.对碳源吸光度做主成分分析(PCA)和单因素方差分析发现微生物多样性梯度的土壤碳源利用模式也存在差异.因此,微生物多样性下降影响土壤的碳矿化速率和碳源利用模式,导致陆地生态系统的功能发生改变,在森林土壤管理中,应重视土壤微生物物种多样性变化对生态系统功能的影响.
[Abstract]:Soil microbial community plays an important role in the ecosystem process, but there is no consistent conclusion on the effect of soil microbial diversity on ecosystem function.In order to study the effect of microbial diversity on soil carbon metabolism characteristics, soil suspensions of Pang Quanggou broadleaved mixed forest were inoculated with sterilized soil samples.The changes of soil carbon mineralization rate and carbon metabolic utilization patterns under different diversity gradients were measured by basic titration and Biolog Eco plate.The results showed that the carbon mineralization rate, cumulative carbon mineralization amount, average pore color change rate and diversity index Shannon Mc Intosh and richness index were significantly higher in D5 treatment than in D5 treatment after 6 weeks of culture.Correlation analysis showed that accumulative carbon mineralization and AWCD were negatively correlated with richness.The soil carbon source utilization patterns of microbial diversity gradient were also found to be different by the principal component analysis (PCAs) and single factor variance analysis (ANOVA) of carbon source absorbance.Therefore, the decline of microbial diversity affects the soil carbon mineralization rate and carbon source utilization patterns, resulting in changes in the function of terrestrial ecosystems, in forest soil management,Attention should be paid to the effect of soil microbial species diversity on ecosystem function.
【作者单位】：山西大学黄土高原研究所;
【基金】：国家自然科学基金项目(41271530)
【分类号】：S154.1

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