干旱条件下钙离子对一氧化氮诱导黄瓜不定根发生的影响

发布时间：2018-04-13 12:45

  本文选题：黄瓜 + 干旱　； 参考：《应用生态学报》2017年11期

【摘要】：以黄瓜品种‘新春4号’为材料,研究干旱胁迫下一氧化氮(NO)和钙离子(Ca~(2+))处理下黄瓜的生根指标、内源Ca~(2+)荧光强度以及抗氧化酶(超氧化物歧化酶SOD、过氧化氢酶CAT、抗坏血酸过氧化物酶APX)活性,分析干旱条件下黄瓜不定根发生过程中NO和Ca~(2+)之间的关系.结果表明:200μmol·L~(-1)氯化钙(CaCl_2)和0.05%聚乙二醇(PEG)共处理显著提高了干旱条件下黄瓜不定根的根长和根数;添加Ca~(2+)螯合剂(EGTA)和通道抑制剂(BAPTA/AM)处理显著降低了干旱条件下NO诱导的不定根根数和根长.干旱条件下,NO和CaCl_2处理提高了黄瓜下胚轴内源Ca~(2+)荧光强度;而NO清除剂(c PTIO)处理的Ca~(2+)荧光强度显著低于NO处理.干旱条件下,NO和CaCl_2处理显著提高了黄瓜下胚轴抗氧化酶活性;而Ca~(2+)抑制剂或螯合剂处理显著降低了NO诱导的抗氧化酶活性.由此可见,干旱条件下Ca~(2+)参与了NO调控黄瓜抗氧化酶活性,缓解了干旱胁迫对不定根形成产生的伤害,进而促进了不定根的发生.
[Abstract]:The rooting index of cucumber (Cucumber cultivar 'Xinchun 4') was studied under drought stress (no) and Ca ~ (2 +) ~ (2 +) treatment.The fluorescence intensity of endogenous Ca~(2 and the activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (cat), ascorbic acid peroxidase (Ca~(2)), and the relationship between no and Ca~(2 during adventitious root formation in cucumber under drought conditions were analyzed.The results showed that the root length and root number of adventitious roots of cucumber under drought condition were significantly increased by the co-treatment of CaCl2) and 0.05% polyethylene glycol (PEG).The addition of Ca~(2) chelating agent and channel inhibitor BAPTA / AM significantly reduced the number of adventitious roots and root length induced by no under drought conditions.The fluorescence intensity of endogenous Ca~(2 of cucumber Hypocotyl was increased by no and CaCl_2 treatments, but the fluorescence intensity of Ca~(2 treated by no scavenger was significantly lower than that of no treatment.The antioxidant enzyme activity of cucumber Hypocotyl was significantly increased by the treatment of no and CaCl_2 under drought condition, while the activity of antioxidant enzyme induced by no was significantly decreased by Ca~(2 inhibitor or chelating agent.It can be seen that Ca~(2) takes part in the regulation of antioxidant enzyme activity in cucumber under drought condition, which alleviates the injury caused by drought stress on adventitious root formation and promotes the formation of adventitious root.
【作者单位】： 甘肃农业大学园艺学院;
【基金】：国家自然科学基金项目(31160398,31560563) 中国博士后科学基金项目(20100470887,2012T50828) 教育部科学技术研究重点项目(211182);教育部高校博士点新教师基金项目(201162020005) 甘肃省自然科学基金项目(1308RJZA179,1606RJZA073)资助~~
【分类号】：S423;S642.2
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本文编号：1744547