秦岭红桦林土壤细菌群落剖面分布特征及其影响因素

发布时间：2018-04-15 10:08

本文选题：红桦林 + 高通量测序　；参考：《环境科学》2017年07期

【摘要】：研究秦岭辛家山林区红桦林细菌群落在土壤剖面上的分布状况,对评估土壤细菌在森林生态系统土壤肥力调节、碳氮循环等作用至关重要.采用Illumina MiSeq高通量测序技术对土壤细菌16S r DNA V3~V4可变区进行测序,结合相关生物信息学分析,初步探讨了红桦林0~10、10~20、20~40和40~60 cm这4个土壤层细菌群落丰富度、多样性指数和细菌群落组成及丰度变化.结果表明,在红桦林土壤剖面上,OTUs、Chao1指数、Shannon指数均在0~10 cm处达到最大值,分别为1 688、2 314、8.66,土层间差异不显著.4个土壤层的优势菌门均为酸杆菌门(Acidobacteria)、变形菌门(Proteobacteria),主要的优势菌属为Gp4、Gp6和Gp16.优势菌门的相对丰度在土层间并不相同,0~10 cm土壤层具有较高的变形菌门(Proteobacteria),其相对丰度为23.62%,而40~60 cm具有较高的酸杆菌门(Acidobacteria),相对丰度为62.88%.酸杆菌门(Acidobacteria)与全氮、土壤有机碳、C/N、可溶性有机碳显著相关,变形菌门(Proteobacteria)与土壤含水量、土壤有机碳、可溶性有机碳显著相关.经RDA分析证明,影响秦岭红桦林土壤剖面细菌群落分布的主要土壤因素是可溶性有机碳.这些研究结果表明在秦岭红桦林土壤4个土层均有较高的细菌多样性,为进一步认识森林土壤细菌多样性奠定了理论基础,在研究森林生态系统土壤剖面养分循环过程时应予以考虑.
[Abstract]:It is very important to study the distribution of bacterial community in the soil profile of the red birch forest in Xinjiashan forest area of Qinling Mountains in order to evaluate the regulation of soil bacterial fertility and the carbon and nitrogen cycle in the forest ecosystem.The 16s r DNA V3~V4 variable region of soil bacteria was sequenced by Illumina MiSeq high-throughput sequencing technique. Combined with the bioinformatics analysis, the richness of bacterial communities in the four soil layers of the red birch forest (01010, 10, 10, 20, 20, 20, 40, 40, 60 cm) was preliminarily studied.Diversity index, composition and abundance of bacterial community.The results showed that the Shannon index of OTUsN Chao1 index reached the maximum at 0 ~ 10 cm on the soil profile of birch forest.The dominant bacteria in the four soil layers were Acidobacteria acidobacteriae, Proteobacteriae, Proteobacteria, and the main dominant bacteria were Gp4Gp6 and Gp16.The relative abundance of dominant phylum was different between soil layers, and the relative abundance of Proteobacteriae was 23.62cm, while that of 400.60cm was higher than that of Acidobacteriae, and the relative abundance was 62.88.Acidobacteria) was significantly correlated with total nitrogen, soil organic carbon C / N, soluble organic carbon, and Proteobacteria) with soil water content, soil organic carbon and soluble organic carbon.RDA analysis showed that soluble organic carbon was the main soil factor that affected the distribution of bacterial community in the soil profile of red birch forest in Qinling Mountains.These results indicated that there was high bacterial diversity in the four soil layers of the red birch forest in the Qinling Mountains, which laid a theoretical foundation for further understanding the bacterial diversity in the forest soil.The nutrient cycling process in soil profile of forest ecosystem should be considered.
【作者单位】：西北农林科技大学资源环境学院农业部西北植物营养与农业环境重点实验室;陕西省宝鸡市辛家山林业局;西北农林科技大学林学院;
【基金】：国家林业公益性行业科研专项(201304307)
【分类号】：S714.3

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