禾谷炭疽菌效应分子motif序列研究

发布时间：2018-04-20 02:02

本文选题：禾谷炭疽菌 + 效应分子　；参考：《科学技术与工程》2016年18期

【摘要】：禾谷炭疽菌侵染玉米、小麦等粮食作物而引起的炭疽病,给各国农业生产造成了巨大经济损失。植物病原菌可以利用效应分子来操控寄主植物的防卫反应,从而实现其对植物的侵染、定殖和扩展等过程。研究基于前人对禾谷炭疽菌中已报道的177个候选效应分子(CE)的氨基酸数据,利用MEME对全部CE和不同氨基酸类别的CE进行在线motif分析,未获得较满意结果。随后,结合已报道效应分子(E)所具有保守motif的氨基酸位置范围,对82个具有典型特征E的信号肽序列后30~60 aa进行获取,并将信号肽位置大于28、氨基酸数量较小的E进行剔除,最终明确64个E存在着保守的PAAX motif。该研究丰富了真菌保守motif的类型,为进一步开展禾谷炭疽菌效应分子的motif功能研究提供重要的理论指导。
[Abstract]:Anthracnose caused by anthracnose of cereal crops such as corn, wheat and so on has caused huge economic losses to agricultural production in various countries. Plant pathogens can use effector molecules to control host plant defense response, and thus to achieve its infection, colonization and expansion of plants. Based on the amino acid data of 177 candidate effector molecules (CEs) reported by previous researchers, MEME was used to analyze all CE and CE of different amino acids on line. The results were not satisfactory. Then, according to the amino acid position range of conserved motif, 82 signal peptide sequences with typical characteristic E were obtained. The signal peptide position was more than 28 and the number of amino acids was small. Finally, it was clear that 64 E had conservative PAAX motif. This study enriches the types of conserved motif of fungi and provides important theoretical guidance for the further study of the motif function of the effector molecules of A. graminearum.
【作者单位】：西南林业大学林学院云南省森林灾害预警与控制重点实验室;廊坊市文安县农业局;
【基金】：国家自然科学基金项目(项31560211) 云南省森林灾害预警与控制重点实验室开放基金项目(项ZK150004) 云南省优势特色重点学科生物学一级学科建设项目(50097505) 云南省高校林下生物资源保护及利用科技创新团队(2014015) 云南省教育厅科学研究基金项目(2014Y330)资助
【分类号】：S432.4

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