黄瓜根际解钾细菌的分离筛

发布时间：2018-04-21 00:35

本文选题：黄瓜 + 解钾细菌　；参考：《北方园艺》2017年13期

【摘要】：以黄瓜根际土壤为试材,采用亚历山大硅酸盐细菌培养基从黄瓜根际土壤中分离筛选解钾菌,用原子吸收法测定菌株的解钾能力,选出解钾能力较强的菌株进行菌种鉴定,同时研究其对黄瓜幼苗生长的影响。结果表明:从黄瓜根际土壤中共分离获得9株细菌,其中PK1、PK3和PK7具有较强的解钾能力,液体培养96h后其解钾率分别为30.05%、33.78%和44.01%。依据菌株的形态学特征和生理生化特征鉴定,PK1和PK3为恶臭假单胞菌(Pseudomones putida),PK7为胶质类芽孢杆菌(Paenibacillus mucilaginosus)。盆栽试验结果表明,与对照相比,14d后PK1提高黄瓜的根长、株高、鲜质量和干质量分别为42.60%、10.45%、48.65%、33.01%,PK3分别对应提高65.26%、21.09%、83.78%和49.48%,PK7分别对应提高79.46%、44.26%、105.41%和113.03%。
[Abstract]:Using cucumber rhizosphere soil as test material, the bacteria were isolated and screened from cucumber rhizosphere soil by Alexandrium silicate culture medium. The ability of decomposition of potassium was determined by atomic absorption spectrometry (AAS), and the strains with strong ability of decomposition of potassium were selected for identification. At the same time, the effect of Cucumber seedling growth was studied. The results showed that 9 strains of bacteria were isolated from cucumber rhizosphere soil, among which PK1 PK3 and PK7 had strong ability to dissolve potassium. After 96 h of liquid culture, the rate of decomposition of potassium was 30.05% and 44.01%, respectively. According to the morphological and physiological and biochemical characteristics of the strain, PK1 and PK3 were identified as Pseudomonas putida, PK7, Paenibacillus mucilaginosus. The results of pot experiment showed that the root length, plant height, fresh weight, fresh weight and dry weight of cucumber were increased by 42.60 and 10.45 after 14 days compared with the control, respectively. PK3 was increased by 65.262621.099.78% and 49.48PK7 by 79.4644.26105.41% and 113.03%, respectively.
【作者单位】：周口师范学院生命科学与农学学院;
【基金】：河南省科技厅科技攻关资助项目(162102310588) 周口师范学院校本资助项目(ZKNUB215210)
【分类号】：S154.3;S642.2

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