油菜亲和性根际菌株的筛选及其促生效果

发布时间：2018-04-22 03:06

本文选题：油菜 + 植物根际促生菌　；参考：《西北农林科技大学学报(自然科学版)》2016年01期

【摘要】：【目的】从油菜根际土壤中筛选油菜亲和性根际菌株,以期获得油菜专用优良特异性根际促生菌。【方法】采用常规稀释平板法从油菜根际筛选油菜根际细菌,提取油菜凝集素进行复筛,获得油菜亲和性根际细菌,测定复筛菌株的产铁载体、吲哚乙酸(IAA)能力,以及1-氨基环丙烷-1-羧酸(ACC)脱氨酶和溶磷活性,采用盆栽试验评价菌株对油菜的促生效果。【结果】经过复筛获得油菜亲和性根际细菌32株,其中30株菌株具有产铁载体能力,16株菌株的产铁载体能力达到5+水平;29株菌株具有溶磷活性,7株菌株产量达到100.00μg/mL;16株菌株能产IAA,菌株P17的产量最高,达到30.48mg/L;7株菌株具有ACC脱氨酶活性。盆栽试验表明,有25株菌株具有不同程度的促生作用,N10和K6促生作用显著,22株菌株的促生作用达到施全肥水平。【结论】采用凝集素作为筛选工具可以快速获得大量与油菜具有特异亲和性的PGPR菌株,菌株N10和K6作为油菜特异性PGPR具有很大的应用潜力。
[Abstract]:[objective] to screen rapeseed affinity rhizosphere strains from rapeseed rhizosphere soil in order to obtain rapeseed specific and specific rhizosphere promoting bacteria. [methods] to screen rapeseed rhizosphere bacteria from rapeseed rhizosphere by conventional dilution plate method. Rapeseed lectin was extracted and screened to obtain rapeseed affinity rhizosphere bacteria. The iron-producing carrier, indole-acetic acid IAA, 1-aminocyclopropane-1-carboxylic acid deaminase and phosphorus-soluble activity were determined. Pot experiment was used to evaluate the growth promoting effect of the strain on rapeseed. [results] 32 rapeseed affinity rhizosphere bacteria were obtained by re-screening. Among them, 30 strains have the ability to produce iron carrier. 16 strains have the ability to produce iron vector. 29 strains have the ability to dissolve phosphorus. The yield of 7 strains can reach 100.00 渭 g / mL, 16 strains can produce IAA, and the yield of P17 strain is the highest. The ACC deaminase activity of 7 strains reached 30.48 mg 路L ~ (-1) L ~ (-1). Pot experiment showed that, There were 25 strains with different degrees of growth promoting effect. 22 strains of N10 and K6 had significant promoting effect on growth. [conclusion] A large number of rapeseed with lectin can be obtained quickly by using lectin as a screening tool. PGPR strains with specific affinity, Strains N10 and K6 have great application potential as rapeseed specific PGPR.
【作者单位】：安徽农业大学生命科学学院;安徽农业大学农学院;
【基金】：国际植物营养研究所项目(IPNI-Anhui-19) 2013年安徽省自主创新科技专项“高油酸油菜新品种选育与生产加工关键技术研究及产业化” 安徽省油菜产业技术体系项目(AHCYTX-04) 安徽省自然科学基金项目(1208085QC62) 安徽省高校青年基金重点项目(2013SQRL015ZD)
【分类号】：S565.4;S154.3

【相似文献】