十字花科黑腐病菌dsbA基因控制的亚细胞蛋白质组分析

发布时间：2018-04-24 18:59

本文选题：Xcc + dsbAA　；参考：《基因组学与应用生物学》2016年06期

【摘要】：本研究以十字花科黑腐病菌(Xarcthomorcas campestris pv.campestris,Xcc)8004菌株为研究对象,采用双向电泳—质谱技术分析比较了dsbA1A2突变体和野生型菌株的分泌蛋白、周质蛋白表达谱。与野生型菌株相比,选取的31个差异蛋白中,14个蛋白点显著上调,17个蛋白点显著下调。对这些蛋白质点进行质谱鉴定和分析,结果显示,dsbA1A2基因的缺失导致了周质蛋白中与β桶结构膜蛋白形成有关的伴侣蛋白SurA的含量降低,几种未知功能的分泌蛋白的分泌量减少以及外膜蛋白OmpW、OmpW1在周质空间的积累。推测这些蛋白质对Xcc 8004的致病性至关重要,为进一步研究Xcc 8004中DSB系统的作用机理提供了依据。
[Abstract]:In this study, Xarcthomorcas campestris pv.campestris Xcc8004 strain was used to analyze and compare the secretory protein and periplasmic protein expression profiles of dsbA1A2 mutant and wild-type strain by two-dimensional electrophoretic mass spectrometry. Compared with wild-type strains, 14 of the 31 differential proteins were up-regulated and 17 were down-regulated. These protein spots were identified and analyzed by mass spectrometry. The results showed that the deletion of dsbA1A2 gene resulted in a decrease in the content of chaperone SurA related to the formation of 尾 -barrel structural membrane protein in periplasmic protein. The secretion of several secreted proteins with unknown function was decreased and the outer membrane protein OmpW1 was accumulated in the periplasmic space. It is suggested that these proteins are important to the pathogenicity of Xcc 8004, which provides a basis for further study on the mechanism of DSB system in Xcc 8004.
【作者单位】：亚热带农业生物资源保护与利用国家重点实验室;广西大学生命科学与技术学院;
【基金】：广西教育厅基金一般项目(KY2015YB012) 广西创新研究团队项目(2014GXNSFFA118005)共同资助
【分类号】：S432.4

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