高通量测序技术下连作植烟土壤细菌群落与土壤环境因子的耦合分析

发布时间：2018-04-25 00:34

本文选题：烤烟 + 连作　；参考：《农业生物技术学报》2016年11期

【摘要】：为了揭示连作条件下植烟土壤细菌群落结构及其与土壤环境间的响应关系,本研究采用了Illumina平台Hiseq2500高通量测序技术,对不同施肥处理(常规施肥,蚯蚓粪肥,微生物菌肥以及蚯蚓粪和微生物菌肥混合)的漯河烟区连作植烟土壤细菌进行16S r RNA V4区测序,结合冗余分析(redundancy analysis,RDA)研究土壤细菌微生物的群落结构组成、多样性以及与土壤环境间的相关关系。结果表明,测序质控后共获得25 203个操作分类单元(operational taxonomic units,OTUs),计1 600 239条读数。分层聚类图显示,不同施肥处理的连作植烟土壤细菌群落有较大差异,但这种差异不体现在结构多样性上。多样性指数分析表明,连作植烟土壤细菌群落易受环境变化的影响,体现出一定的时间差异性;烤烟成熟期土壤丰度指数明显升高,微生物菌肥和蚯蚓粪肥处理下土壤细菌群落丰度变化较大。主成分分析表明,不同土壤环境因子间有很强的相关关系,可以将原有的11个土壤环境因子按照强正相关关系划分为4类;RDA结果表明,土壤p H既影响土壤细菌群落的多样性,又影响土壤细菌群落的丰度;而有机质主要对土壤细菌群落丰度有积极影响。研究结果为在微生物水平上研究连作植烟障碍的形成机理提供了依据。
[Abstract]:In order to reveal the community structure of soil bacteria and its response to soil environment under continuous cropping conditions, Hiseq2500 high-throughput sequencing technique based on Illumina platform was used to treat different fertilization treatments (conventional fertilization and earthworm manure). Microbial manure, earthworm manure and microbial fertilizer were used to sequence the 16s r RNA V4 region of soil bacteria in Luohe tobacco area, and the community structure of soil bacteria was studied by redundancy analysis. Diversity and correlation with soil environment. The results showed that a total of 25,203 operational taxonomic units were obtained after quality control, with a total of 1 600239 readings. Stratified cluster map showed that the bacterial communities of tobacco planting soils with different fertilization treatments were different, but the differences were not reflected in structural diversity. The analysis of diversity index showed that the bacterial community of continuous cropping tobacco planting soil was easy to be affected by environmental changes, and the soil abundance index of flue-cured tobacco at mature stage was obviously increased. Microbial fertilizer and earthworm manure treatment had a great change in the abundance of soil bacterial community. Principal component analysis (PCA) showed that there was a strong correlation between different soil environmental factors. According to the strong positive correlation, 11 soil environmental factors could be divided into 4 classes of RDA. The results showed that soil pH affected the diversity of soil bacterial community. The abundance of soil bacterial community was also affected by organic matter, and organic matter had a positive effect on the abundance of soil bacterial community. The results provide a basis for studying the formation mechanism of tobacco barrier in continuous cropping at microbial level.
【作者单位】：河南农业大学烟草学院;河南省烟草公司漯河市公司;
【基金】：中国烟草总公司河南省公司重点科技攻关项目(No.HYKJ201503)
【分类号】：S572;S154.3

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