龙门山地震带土壤细菌多样性的研究

发布时间：2018-04-25 09:34

本文选题：变性梯度凝胶电泳 + 龙门山地震带　；参考：《土壤通报》2017年05期

【摘要】：采用16S r DNA变性梯度凝胶电泳(Denaturing Gradient Gel Electrophoresis,DGGE)技术,结合不同土壤样品的理化性质差异,分析龙门山地震带土壤细菌多样性。结果表明,不同的土壤样品其理化性质存在着明显的差异,三种植被类型的土壤(杂木林、竹林和松树林)中,杂木林的土壤pH值和全氮含量最高,竹林最低;而土壤碱解氮、速效磷、速效钾和有机质含量则是杂木林比竹林高,松树林最低。对DGGE优势条带测序分析表明,回收的11个优势条带(条带A~K)中,条带A、C、D、F、G和H属于变形菌门,条带E、J和K属于酸杆菌门,条带B属于放线菌门,条带I属于装甲菌门,变形菌门占细菌总类群的54.55%,说明龙门山地震带土壤优势菌群为变形菌门细菌。细菌群落多样性与土壤理化性质相关性的统计分析结果显示,影响细菌群落多样性的主要环境因子在杂木林土壤中为速效钾(r=0.80,P0.05),竹林为有机质(r=0.97,P0.05),松树林则是pH值(r=0.35,P0.05),说明龙门山地震带不同植被类型土壤中的细菌群落对土壤理化性质的需求不同。由于地震能够破坏土壤结构,改变土壤理化性质,从而导致土壤细菌群落多样性发生改变,因此,研究龙门山地震带土壤细菌多样性为震后灾区重建工作和维持龙门山地震带生态系统的可持续性提供了科学依据。
[Abstract]:The bacterial diversity in Longmen Mountain seismic zone was analyzed by 16s r DNA denaturing Gradient Gel gel electrophoresis (DGGE) technique and the physical and chemical properties of different soil samples. The results showed that there were obvious differences in physical and chemical properties of different soil samples. Among the three vegetation types of soil (hybrid forest, bamboo forest and pine forest), the soil pH value and total nitrogen content of hybrid forest were the highest, and that of bamboo forest was the lowest, while that of soil alkali-hydrolyzed nitrogen was the lowest. The contents of available phosphorus, available potassium and organic matter were higher in the hybrid forest than in the bamboo forest, and the lowest in the pine forest. The sequence analysis of DGGE dominant bands showed that among the 11 reclaimed dominant bands (AK), the bands ACU, D, F, G and H belonged to Proteus, bands EJ and K belonged to acidobacterium, strip B belonged to actinomycetes, and strip I belonged to armored bacteria. Proteus accounted for 54.55% of the total bacterial group, indicating that the dominant soil flora of Longmen Mountain seismic belt was Proteus. The results of statistical analysis on the correlation between bacterial community diversity and soil physical and chemical properties showed that, The main environmental factors that affect the diversity of bacterial community are available potassium 0.80 and P0.05 in the soil of hybrid forest, organic matter of 0.97 and P0.05 in bamboo forest, and pH value of 0.35 and P0.05in the pine forest, indicating that the bacterial community in different vegetation types of soil in Longmen Mountain seismic belt is physical and chemical to the soil. The nature of the demand is different. Because earthquakes can destroy soil structure and change soil physical and chemical properties, resulting in soil bacterial community diversity change, therefore, The study of soil bacterial diversity in Longmen Mountain earthquake zone provides a scientific basis for rebuilding the disaster area after the earthquake and maintaining the sustainability of the ecological system in the Longmen Mountain seismic belt.
【作者单位】：西华师范大学生命科学学院西南野生动植物资源保护省部共建教育部重点实验室;西华师范大学环境科学与工程学院;
【基金】：生态安全与保护四川省重点实验室开放基金资助项目(ESP201307)资助
【分类号】：S714.3

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