保护地黄瓜根内和土壤中丛枝菌根真菌和深色有隔内生真菌多样性研究（英文）

发布时间：2018-04-28 11:46

本文选题：丛枝菌根真菌 + 深色有隔内生真菌　；参考：《菌物学报》2017年02期

【摘要】：对于野生植物根内定殖的丛枝菌根真菌(AMF)和暗隔内生真菌(DSE)多样性及其生态功能现已进行了众多的调查研究。然而,对于同时定殖于栽培作物同一根系的这两种真菌的物种多样性和功能了解甚少。本研究旨在采用传统的形态学方法和PCR DGGE技术探究保护地栽培的黄瓜Cucumis sativus Linn.根内AMF和DSE的物种多样性。PCR DGGE结果显示共有7种AMF,包括Funneliformis mosseae,Glomus fasciculatum,Glomus indicum,Scutellospora dipurpurescens,Gigaspora margarita以及2个未培养的Archaeospora;而以黄瓜植株根段作为接种物加富培养后,依据其所产生的孢子形态特征进行分类鉴定,则只分离获得3种,即F.mosseae,G.indicum和Gi.Margarita;同时,采用常规分离纯化的方法从黄瓜根内分离得到DSE 6个菌株,其中1株经分子生物学鉴定为Phoma leveillei。从保护地根区土壤中分离AMF孢子并通过形态学分类鉴定,获得了9属20种。研究结果表明,Glomus是保护地栽培黄瓜根系内的优势属,针对数量,相对于传统形态鉴定技术,分子技术可以检测到根内更多的AMF。
[Abstract]:The diversity and ecological function of arbuscular mycorrhizal fungi AMF and DSEs of wild plants have been investigated. However, little is known about the species diversity and function of the two fungi that colonize the same root system at the same time. The purpose of this study was to explore the cultivation of cucumber Cucumis sativus Linn by traditional morphological method and PCR DGGE technique. Species diversity of AMF and DSE in root. DGGE results showed that there were 7 species of AMFs, including Funneliformis mosseaeae Glomus fasciculatumus Glomus indicum Scutellospora dipurpurescensen Gigaspora margarita and two uncultured Archaeospora. According to the morphological characteristics of the spores produced, only 3 species were isolated, I. E. F. mosseaeum G. indicum and Gi.Margarita, and six strains of DSE were isolated from cucumber roots by routine isolation and purification, and one of them was identified as Phoma leveillei by molecular biology. AMF spores were isolated from the soil of protected root zone and identified by morphological taxonomy, and 20 species belonging to 9 genera were obtained. The results showed that Glomus was the dominant genus in the root system of protected cucumber. Compared with the traditional morphological identification technique, the molecular technique could detect more AMFs in the roots of cucumber.
【作者单位】：青岛农业大学菌根生物技术研究所;中国科学院微生物研究所真菌学国家重点实验室;
【基金】：Supported by the National Natural Science Foundation of China(31470101,31272210) the Basic Research Project of Qingdao Government[12 1 4 5 (14) jch]
【分类号】：S626;S642.2;S182

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