不同施肥措施对番茄连作土壤酚酸含量和微生物功能多样性的调节

发布时间：2018-05-05 17:37

本文选题：鸡粪 + 稻草　；参考：《土壤通报》2017年04期

【摘要】：设施内蔬菜连作土壤酚酸含量和微生物功能多样性的异常是导致连作障碍的关键因素。利用毛细管电泳及BiologECO生理学研究法研究了鸡粪(M)、鸡粪+K2SO4(MK)、鸡粪+稻草(MR)、鸡粪+稻草+K2SO4(MRK)对温室内番茄连作6年土壤中对羟基苯甲酸、苯甲酸、阿魏酸及肉桂酸及微生物功能多样性的影响,以不施肥处理为对照(CK)。结果表明:与CK相比,不同物料组合施用能够显著降低土壤中酚酸含量,其中MRK处理降低效果最明显,土壤中对羟基苯甲酸、苯甲酸分别降低7.11%~57.04%、81.84%~83.32%。不同物料组合施用提高了土壤微生物对碳源的利用强度,其中MRK处理土壤微生物对碳源利用能力最强,且微生物多样性指数最高,其Shannon和Mch Intosh多样性指数比其他处理平均提高3.93%~10.07%、6.22%~27.47%。主成分分析表明,M处理土壤的微生物功能多样性差异主要是由于氨基酸、羧酸类、聚合物或酚酸类碳源引起的,而MR处理则是由碳水化合物或胺类碳源引起。微生物多样性指数与土壤中对羟基苯甲酸、苯甲酸、阿魏酸及肉桂酸含量呈显著负相关。MRK处理番茄产量分别比MK、MR、M、CK提高5.87%、11.34%、22.85%、30.13%,但MK番茄产量与MRK相差不明显。设施栽培条件下,鸡粪、稻草和K2SO4配合施用对提高土壤微生物功能多样性,降低土壤酚酸类物质含量,减缓连作障碍具有重要意义。
[Abstract]:The abnormal phenolic acid content and microbial functional diversity are the key factors causing the continuous cropping obstacle. Capillary electrophoresis (CE) and BiologECO physiology were used to study the effects of chicken manure, and chicken manure, K _ 2SO _ 4 and K _ 2SO _ 4 on the treatment of p-hydroxybenzoic acid in tomato soil in greenhouse for six years. The effects of ferulic acid, cinnamic acid and microbial functional diversity were studied. The results showed that the content of phenolic acid in soil was significantly decreased by applying different combinations of materials compared with CK, among which, the effect of MRK treatment was the most obvious, and the contents of p-hydroxybenzoic acid and benzoic acid in soil decreased 7.11%, 57.04% and 83.32% respectively. The application of different combinations of materials improved the soil microbial utilization intensity of carbon source, and the ability of soil microorganism to use carbon source was the strongest, and the microbial diversity index was the highest. The Shannon and Mch Intosh diversity index increased by 3.93% and 10.07%, 6.22% and 27.47%, respectively, compared with other treatments. Principal component analysis (PCA) showed that the diversity of microbial function was mainly caused by amino acid, carboxylic acid, polymer or phenolic acid carbon source, while Mr treatment was caused by carbohydrate or amine carbon source. The microbial diversity index was significantly negatively correlated with the contents of p-hydroxybenzoic acid, ferulic acid and cinnamic acid in soil. The yield of tomato treated with MRK was 5.87% higher than that of MK, 11.34% and 22.85% (30.13%), respectively, but the yield of MK tomato was not significantly different from that of MRK. The combination of chicken manure, straw and K2SO4 could improve the functional diversity of soil microorganisms, reduce the content of phenolic acids in soil and slow down the obstacle of continuous cropping.
【作者单位】：沈阳农业大学土地与环境学院;
【基金】：国家自然科学基金(31372132) 国家重点研发计划项目(2016YFD0201004) 教育部博士点基金(20132103110008)资助
【分类号】：S154.3

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