黄瓜根际芽孢杆菌亲缘辨识特性研究

发布时间：2018-05-06 23:30

本文选题：亲缘辨识 + 群集运动　；参考：《南京农业大学学报》2017年06期

【摘要】：[目的]依据群集运动表型和16S rRNA基因序列信息研究黄瓜根际芽孢杆菌亲缘辨识能力,进而形成直接合作的多细胞行为,找到解淀粉芽孢杆菌SQR9的近亲菌株。[方法]采用高温处理和稀释涂布的方法从黄瓜根际筛选芽孢杆菌,得到菌株后分析其16S rRNA基因测序结果,结合swarming平板对峙表型对芽孢杆菌类群进行亚家族分类。最后通过24孔细胞培养板静置培养、salkowski比色法分析吲哚乙酸(IAA)产量和植酸酶活性测定的方法评价SQR9近亲菌株的促生潜力。[结果]初筛共选出118株芽孢杆菌,复筛后确定为21株。菌株swarming对峙后表现融合、半融合和对峙3种表型。在融合的27组菌株里,25组有100%相同的16S rRNA基因,而其余2组的基因相似度较低。产生边界的组合中只有10%(142对中只有15对)的16S rRNA基因相似度大于99%;半融合的基因相似性为99.5%~100%。菌株SQR9的亲近菌株P2、W-4和N-2在生物膜形成、IAA分泌和植酸酶活性等方面都接近SQR9。[结论]初步证明swarming表型与16S rRNA有一定的相关性,菌株SQR9近亲菌株P2、W-4和N-2都是潜在的根际促生菌。
[Abstract]:[objective] to study the genetic identification ability of Bacillus rhizosphere in cucumber based on the cluster motion surface and 16s rRNA gene sequence information, and to form a direct cooperative multicellular behavior, and to find a close relative strain of Bacillus amylolyticus SQR9. [methods] Bacillus spp. Was screened from cucumber rhizosphere by high temperature treatment and dilution coating. The 16s rRNA gene sequencing results were analyzed, and the subfamily classification of Bacillus subfamily was carried out combined with the phenotype of swarming plate confrontation. Finally, the growth promotion potential of SQR9 indole-acetic acid (SQR9) indole-acetic acid (SQR9) indole-acetic acid (SQR9) indole-acetic acid was evaluated by the method of 24-well cell culture plate static culture with salkowski colorimetry. [results] 118 strains of Bacillus were selected and 21 strains were screened again. The strain swarming showed fusion, semi-fusion and confrontation phenotypes after confrontation. There were 100% identical 16s rRNA genes in 25 groups of fusion 27 strains, while the other two groups had lower gene similarity. The similarity of 16s rRNA gene in the combination of 10 pairs and 142 pairs is greater than that of 99%, while the similarity of semi-fusion gene is 99.5%. The close strains P2OW-4 and N-2 of strain SQR9 were close to SQR9 in the production of biofilm and the activity of phytase. [conclusion] the phenotype of swarming was correlated with 16s rRNA, and P2CW-4 and N-2 were both potential rhizosphere growth-promoting bacteria.
【作者单位】：南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化高技术研究重点实验室;江苏省耕地质量与农业环境保护站;
【基金】：国家自然科学基金青年基金项目(31501833) 江苏省自然科学基因青年基金项目(BK20150658) 江苏高校品牌专业建设工程资助项目(PPZY2015A061)
【分类号】：S154.3;S642.2

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