贡嘎山海拔梯度上不同植被类型土壤甲烷氧化菌群落结构及多样性

发布时间：2018-05-09 21:34

本文选题：甲烷氧化菌群落 + 植被类型　；参考：《应用生态学报》2017年03期

【摘要】：甲烷是仅次于CO_2的第二大温室气体.森林表层土壤中甲烷好氧氧化作用是大气甲烷重要的汇,在碳循环和减缓全球变暖方面起着重要作用.研究不同植被类型土壤中甲烷氧化菌的群落结构及多样性,有助于更好地理解植被演替、人为干扰和不同土地利用背景下甲烷氧化菌群落组成和多样性变化与地上植被之间的相互关系.本研究在贡嘎山东坡海拔梯度上的4种不同植被类型中采集了92个土壤样品,利用Miseq测序技术和生物信息学方法评估了甲烷氧化菌群落组成及多样性在4种不同植被类型间的变化,并探讨了其变异的潜在原因.结果表明:常绿阔叶林和针阔叶混交林土壤中甲烷氧化菌的群落结构较为相似,暗针叶林和灌丛草甸土壤甲烷氧化菌的群落结构较为相似.4种不同植被生态系统中,针阔叶混交林土壤中的甲烷氧化菌α多样性显著高于其他3种植被生态系统(P0.001),且暗针叶林和灌丛草甸土壤中甲烷氧化菌β多样性显著高于常绿阔叶林和针阔叶混交林(P0.001).Spearman相关分析表明,不同类型甲烷氧化菌的相对丰度对环境变化的响应模式不同.造成α多样性差异的主要因子可能是土壤总氮、电导率和土壤温度.偏Mantel检验分析和冗余分析(RDA)表明,常绿阔叶林和针阔叶混交林土壤甲烷氧化菌多样性受环境因子的影响较大,而暗针叶林和灌丛草甸土壤中甲烷氧化细菌多样性变化可能存在其他潜在的影响因素或者机制.降水可能是造成低海拔常绿阔叶林和针阔叶混交林与高海拔暗针叶林和灌丛草甸土壤甲烷氧化细菌群落结构差异的主要原因.贡嘎山海拔梯度上不同植被类型土壤中甲烷氧化菌的群落结构和多样性变化可能主要是由于土壤理化性质和气候变化综合作用的结果.
[Abstract]:Methane is the second largest greenhouse gas after CO_2. Aerobic oxidation of methane in forest surface soil is an important sink of atmospheric methane, which plays an important role in carbon cycle and global warming mitigation. The study of the community structure and diversity of methanophore in soils of different vegetation types will contribute to a better understanding of vegetation succession. The relationship between the composition and diversity of CH4 oxidizing bacteria community and the aboveground vegetation in different land use background and anthropogenic disturbance. In this study, 92 soil samples were collected from 4 different vegetation types on the east slope of Gongga Mountain. Miseq sequencing and bioinformatics methods were used to evaluate the changes of CH4 oxidant community composition and diversity among four different vegetation types, and the potential causes of the variation were discussed. The results showed that the community structure of methanophore in evergreen broadleaved forest and coniferous broadleaved mixed forest was similar, and that in dark coniferous forest and shrub meadow soil was similar to that in different vegetation ecosystem. The 伪 -diversity of methanophore in the soil of coniferous broad-leaved mixed forest was significantly higher than that of the other three planting ecosystems (P0.001C), and the 尾 -diversity of methanophore in dark coniferous forest and shrub meadow soil was significantly higher than that in evergreen broad-leaved forest and coniferous broad-leaved mixed forest. The correlation analysis of P0.001N. Spearman showed that, The response patterns of the relative abundance of different methanogens to environmental changes were different. The main factors contributing to the difference of 伪 -diversity may be soil total nitrogen, electrical conductivity and soil temperature. Partial Mantel test and redundancy analysis showed that the diversity of methanogens in evergreen broad-leaved forest and coniferous broad-leaved mixed forest was influenced by environmental factors. There may be other potential influencing factors or mechanisms for the diversity of methanodized bacteria in dark coniferous forest and scrub meadow soil. Precipitation may be the main reason for the difference of methanogenic bacteria community structure between low altitude evergreen broadleaved forest and coniferous broadleaved mixed forest and high altitude dark coniferous forest and shrub meadow soil. The community structure and diversity of methanophore in soils of different vegetation types on the elevation gradient of Gongga Mountain may be mainly due to the comprehensive effects of soil physical and chemical properties and climate change.
【作者单位】：中国科学院成都生物研究所环境与应用微生物重点实验室;中国科学院成都生物研究所环境微生物四川省重点实验室;中国科学院大学;
【基金】：国家自然科学基金项目(31570496,31400458)资助~~
【分类号】：S714.3

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