混合菌群发酵马尾藻作为海藻肥料的研究

发布时间：2018-05-12 05:20

本文选题：发酵 + 马尾藻　；参考：《海洋科学》2017年02期

【摘要】：从马尾藻原位分离发酵菌群,采用分子生物学手段分析鉴定菌种组成,获得发酵菌株4株分别为巨大芽孢杆菌(Bacillus megaterium),非脱羧勒克氏菌(Leclerciaadecarboxylata),鞘氨醇单胞菌(Sphingomonasleidyi)以及赖氨酸芽孢杆菌(Lysinibacillusmacroides);控制4菌株接种比例进行马尾藻发酵,监测发酵过程中菌种生物量、褐藻酸含量及褐藻寡糖含量变化规律,结果表明,马尾藻经发酵36 h生成聚合度2-5的褐藻寡糖,并伴随菌种二次生长及pH下降等易于观察检测的参数变化;含褐藻寡糖的发酵原液稀释600倍后,能够明显促进小麦种子萌发及根生长,与清水对照相比萌发率提高26%,根系长度增长37%。本研究为微生物发酵法生产海藻肥料提供了可行案例,证实该方法简单有效,可操作性强,有广阔的应用前景,为将来实现海藻发酵肥工业化生产提供理论支持和数据支撑。
[Abstract]:The fermentation flora was isolated from Sargassum sargassum in situ and its composition was identified by molecular biological analysis. Four fermentation strains, Bacillus megateriumum, Leclerciaadecarboxylatae, Sphingomonas leidyi) and Lysinibacillus macroides. were obtained. The changes of alginic acid content and alginate oligosaccharide content were studied. The results showed that Sargassum spp. After fermentation for 36 h, it produced alginate oligosaccharide with polymerization degree 2-5, and the change of parameters which was easy to observe and detect was accompanied by the secondary growth of strain and the decrease of pH value. When the fermentation medium containing brown algae oligosaccharide was diluted for 600 times, the germination rate and root length of wheat seed germination and root growth were increased by 26% and 37%, respectively. This study provides a feasible case for microorganism fermentation to produce seaweed fertilizer. It is proved that this method is simple and effective, and has a broad prospect of application. It provides theoretical support and data support for the industrial production of seaweed fermenting fertilizer in the future.
【作者单位】：哈尔滨工业大学市政环境工程学院;中国科学院烟台海岸带研究所;五洲丰农业科技有限公司;哈尔滨工业大学海洋科学与技术学院;
【基金】：威海市科技发展研究计划重点项目(2010-3-96) 国家“十二五”科技支撑计划项目(2013BAB01B0) 中国科学院科技服务网络计划项目(KFJ-EW-STS-060) 国家自然科学基金青年基金项目(41401285) 河口与海岸学国家重点实验室开放课题项目(SKLEC-KF201412) 海洋公益性行业科研项目(201505022)~~
【分类号】：TQ446;TQ920.6

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