枸杞根际土壤真菌群落多样性的高通量测序

发布时间：2018-05-19 17:16

本文选题：枸杞 + 根际土壤　；参考：《微生物学报》2017年07期

【摘要】：【目的】为了解不同枸杞产区枸杞根际真菌多样性的差异,从枸杞根际土壤真菌群落的角度试图解释宁夏枸杞优良品质与其土壤微生物菌群的联系。【方法】采用Mi Seq高通量测序方法,分别将我国4个不同地区枸杞根际土壤样品进行ITS区的高通量测序,并分析了物种组成和丰度、Alpha多样性、Beta多样性和菌群结构,测定不同产区枸杞主要有效成分,同时对枸杞品质和其土壤理化因子及枸杞根际真菌种群多样性的相关性做了分析。【结果】多糖含量和甜菜碱含量均是中宁枸杞高于兴仁和精河,格尔木含量最低;宁夏兴仁、宁夏中宁、新疆精河3个地区菌群结构类似,子囊菌门和结合菌门占总菌群的80%左右,而青海格尔木除了子囊菌门占58%外,壶菌门和新丽鞭毛菌门远远高于其他3个地区,比例占总菌群的近25%;4个地区枸杞根际土壤真菌菌群结构相似性依次为宁夏兴仁、新疆精河、宁夏中宁、青海格尔木。【结论】枸杞根际土壤样品真菌物种组成丰富,不同地区枸杞根际土壤真菌种群结构有一定差异;枸杞主要有效成分含量与枸杞根际土壤真菌种群结构具有一定相关性。
[Abstract]:[objective] to understand the diversity of fungi in rhizosphere of Lycium barbarum in different areas of Lycium barbarum. From the point of view of rhizosphere soil fungi community of Lycium barbarum L., this paper attempts to explain the relationship between the fine quality of Lycium barbarum L. and its soil microbial flora. [methods] Mi Seq high-throughput sequencing method was used. The high-throughput sequencing of Lycium barbarum rhizosphere soil samples from four different regions of China was carried out, and the species composition, abundance Alpha diversity, Beta diversity and microflora structure were analyzed, and the main effective components of Lycium barbarum in different producing areas were determined. The correlation between the quality of Lycium barbarum and soil physical and chemical factors and the diversity of fungi population in rhizosphere of Lycium barbarum was analyzed. [results] the contents of polysaccharides and betaine were higher in Zhongning wolfberry than in Xingren and Jinghe, and the lowest in Golmud. In Zhongning, Ningxia and Jinghe, Xinjiang, the flora structure was similar. The ascomycetes and conjugate accounted for about 80% of the total flora. In addition to the ascomycetes, Gelmu in Qinghai was 58%, and the phylum and flagellate phylum were much higher than those in the other three regions. The structure similarity of fungi in rhizosphere soil of Lycium barbarum L. in four regions was Xingren, Jinghe, Zhongning and Golmud in Qinghai, respectively. [conclusion] the species composition of Lycium barbarum rhizosphere soil is abundant. The population structure of Lycium barbarum rhizosphere fungi was different in different areas, and the main effective components of Lycium barbarum were correlated with the soil fungal population structure of Lycium barbarum rhizosphere.
【作者单位】：宁夏农林科学院枸杞工程技术研究所;石河子大学生命科学学院;石河子大学农学院;
【基金】：宁夏回族自治区农业育种专项(2013NYYZ0102) 国家重大基础研究专项(2016YFC0501404)~~
【分类号】：S154.3

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