不同发育阶段杉木人工林土壤细菌类群特征

发布时间：2018-05-21 12:42

本文选题：杉木 + 人工林　；参考：《西南林业大学学报(自然科学)》2017年05期

【摘要】：采用16S rDNA建库和高通量测序技术对3个不同发育阶段杉木人工林的土壤细菌类群进行分析,探讨杉木人工林不同发育阶段对土壤细菌类群的影响。结果表明:16S rDNA在目水平上鉴定比例为80%以上,结果较可靠;不同发育阶段杉木人工林土壤共鉴定细菌门39个、纲100个、目151个,不同发育阶段之间3种功能性指数均无显著差异,且与部分化学性质相关性较小;其中酸杆菌门、变形菌门、放线菌门、绿弯菌门、疣微菌门为最主要细菌门类。酸杆菌门丰度在不同发育阶段中排序为:幼龄林中龄林老龄林;按丰度变形菌门的排序则为:幼龄林中龄林老龄林。从beta指数分析可知:幼龄林内部相似度相比中、老龄林较低;中、老龄林之间相似度较高,幼龄林与其他2种发育阶段之间相对相似度较低。
[Abstract]:16s rDNA library and high-throughput sequencing were used to analyze the soil bacterial groups in three Chinese fir plantations at different developmental stages, and the effects of different development stages on soil bacterial groups were discussed. The results showed that the proportion of ~ 16s rDNA was more than 80% at the order level, and the results were reliable, and there were 39 phylum, 100 classes and 151 orders in the soil of Chinese fir plantation at different stages of development. There was no significant difference in the three functional indices among the three stages of development, and there was little correlation with some chemical properties, among which, actinomycetes, Proteus, actinomycetes, phylum verrucosa, phylum actinomycetum, phylum verrucosa were the most important bacteria phylum. The order of abundance of acidobacterium phylum in different stages of development was as follows: the middle aged forest of young forest and the old forest of middle age forest of young forest according to the abundance of Proteomycetes phylum. The analysis of beta index showed that the internal similarity of young forest was lower than that of old forest, and the relative similarity between young forest and other two stages of development was lower.
【作者单位】：福建农林大学林学院;国家林业局杉木工程技术研究中心;张北县第一中学;福建农林大学莘口教学林场;
【基金】：国家科技支撑计划项目(2015BAD09B010102)资助国家重点研发计划项目(2016YFD0600301)资助
【分类号】：S714.3

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