三七收获后不同年限土壤微生物代谢多样性分析

发布时间：2018-05-22 07:53

本文选题：三七 + 连作障碍　；参考：《中国生态农业学报》2016年03期

【摘要】：采用Biolog技术,对云南省砚山县的盘龙、阿猛、干河3个乡镇进行了三七历年种植地的调查研究,以未种植过三七的土壤为对照,研究三七收获后1~6 a不同年限对土壤养分、土壤微生物对碳源利用以及土壤微生物多样性的影响。结果表明,反映土壤微生物活性的平均颜色变化率(AWCD)并未随年限增加呈现明显变化规律;微生物培养96 h活性旺盛。对Biolog板31种碳源吸光度值聚类分析表明,3个乡镇所取三七收获后1 a和6 a土壤微生物碳源利用均可聚为一类,其土壤微生物碳源利用特征相似,其余不同年限土壤微生物碳源利用聚类并未出现一定规律;与未种植过三七的土壤相比,三七收获后1 a、6 a土壤微生物均对碳水化合物类、聚合物类、羧酸类和酚类碳源的利用分别高出25.97%~55.59%和53.14%~65.68%;随着三七收获后年限的增加,土壤微生物对碳水化合物、氨基酸类、羧酸类碳源利用呈收获后2 a升高、4 a和5 a下降趋势。收获三七后1~6 a土壤与未种植过三七的土壤相比,氮、磷、钾及有机质含量差异均不显著。除干河乡外,阿猛乡和盘龙乡收获三七后1~6 a土壤微生物与未种植过三七的相比,Shannon-Wiener指数、丰富度指数、Simpson指数均值总体差异不明显。试验说明三七轮作至少需6 a以上时间,土壤微生物对不同碳源的优势利用可反映出三七连作障碍与土壤微生物群落结构差异有密切联系。
[Abstract]:Using Biolog technology, 3 villages and towns of Panlong, ah Meng, and dry river in Yanshan County of Yunnan province were investigated and studied for 37 years. The effects of different years of 1~6 a on soil nutrients, soil microbial utilization and soil microbial diversity after 37 harvest were studied. The average color change rate of soil microorganism activity (AWCD) did not change obviously with the increase of life, and the activity of microbial culture 96 h was exuberant. The cluster analysis of 31 carbon source absorbance values of Biolog plate showed that 1 A and 6 a soil of 3 villages and towns could be used as a kind of soil microbial carbon source. With the similar characteristics, the soil microbial carbon source of the other different years did not appear a certain rule. Compared with the soil without over 37, 1 A after 37 harvest and 6 a soil microbial biomass of carbohydrates, polymers, carboxylic acids and phenols were higher than 25.97%~55.59% and 53.14%~65.68%, respectively, with 37 harvest. After the increase of the post harvest, soil microbial biomass of carbohydrates, amino acids and carboxylic acids increased 2 a after harvest, 4 A and 5 a. After 37 1~6 a soil, the nitrogen, phosphorus, potassium and organic matter content of 1~6 a soil were not significantly different. In addition to Dry River Township, and Pan Long Township after 37 1~6 a harvest. The total difference of Shannon-Wiener index, richness index and Simpson index was not obvious compared with the soil microorganism, which showed that the average difference of Shannon-Wiener index, richness index and Simpson index was at least 6 a time. The advantages of soil microorganism to different carbon sources could reflect the close relationship between the 37 continuous cropping obstacle and the soil microbial community structure difference.
【作者单位】：云南农业大学植物保护学院;
【基金】：云南省发展和改革委员会重大专项项目(20112513)资助~~
【分类号】：S567.236;S154.3

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