细菌菌体对白浆土腐殖质转化的影响

发布时间：2018-06-02 04:54

本文选题：白浆土 + 腐殖质转化　；参考：《水土保持研究》2017年04期

【摘要】：采用白浆土为供试材料,利用混皿法对白浆土土样进行筛菌,将筛选得到的优势菌种进行发酵,设置1%,3%,5%三种不同比例的菌体接种量,在相同条件下与培养好的白浆土进行共培养,探究细菌菌体对白浆土中水溶性腐殖质转化的影响,为土壤腐殖质转化的微生物学机理研究和土壤有机培肥研究提供理论基础。结果显示:(1)各处理WSS均表现为先降低后升高的趋势。(2)其中添加1%细菌菌体的处理有机质含量波动最为平缓,先降低后增加,添加3%细菌菌体的处理在30d开始有机质含量不断增加。而添加5%细菌菌体,有机质含量在30d开始增加,在60d开始下降,各处理的土样中,有机质含量在120d内始终高于对照水平。(3)各处理的土壤当中的HE含量都是在加入细菌菌体后明显增加,然后下降,其中添加3%细菌菌体处理中的HE含量变化最为明显。各处理的HA含量都是不断降低,其中添加1%和3%菌体的处理中HA含量降低速度快。培养结束后,各处理FA含量变化不明显。(4)培养初期,细菌菌体的繁殖促使各处理土样中的微生物数量增大,然后降低,最后趋于平缓。微生物量碳的相对含量在添加不同比例的细菌菌体后均高于对照组,且添加1%细菌菌体的处理组微生物量碳的相对含量最高。
[Abstract]:The white soil was used as the test material, and the sample was screened by mixed dish method. The dominant bacteria were fermented, and the inoculation amount of 3 kinds of bacteria in different proportion was set up. The effects of bacteria on the transformation of water-soluble humus in the soil were investigated by co-culture with the cultivated alpine soil under the same conditions, which provided a theoretical basis for the study of the microbiological mechanism of soil humus transformation and the study of soil organic fertilization. The results showed that the WSS of each treatment decreased first and then increased. The content of organic matter increased continuously at 30 days after treatment with 3% bacteria. The content of organic matter increased at 30 days and decreased at 60 days after adding 5% bacteria. In 120 days, the content of organic matter in the soil was higher than that in the control level. The content of HE in the soil of each treatment increased obviously after adding bacteria, and then decreased, among which the content of HE in the treatment of 3% bacteria was the most obvious. The HA content of each treatment decreased continuously, and the HA content decreased rapidly in the treatment of adding 1% and 3% bacteria. After the end of culture, the FA content of each treatment did not change obviously. 4) at the initial stage of culture, the propagation of bacteria resulted in the increase of the number of microbes in each treatment soil sample, then decreased, and finally tended to flat. The relative content of microbial biomass carbon was higher than that of the control group after adding different proportion of bacteria, and the relative content of microbial biomass carbon was the highest in the treatment of adding 1% bacteria.
【作者单位】：吉林农业大学资源与环境学院;吉林省嘉博生物科技有限公司;
【基金】：国家基础研究计划项目(2009CB426308) 吉林省科技厅项目(20130206018NY) 土壤与农业可持续发展国家重点实验室开放课题项目(Y412201454)
【分类号】：S153.622

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