有机碳氮添加对酸性森林土壤氨氧化过程的影响

发布时间：2018-06-03 09:13

本文选题：氨氧化古菌 + γ-氨基丁酸　；参考：《土壤学报》2017年04期

【摘要】：以亚热带酸性森林土壤为研究对象,开展了微宇宙室内培养实验,设置了有机碳和有机氮添加处理,分析了土壤硝化活性和氨氧化古菌(Ammonia-oxidizing archaea,AOA)、氨氧化细菌(Ammonia-oxidizing bacteria,AOB)的功能基因丰度,研究了外源有机碳和有机氮对酸性森林土壤氨氧化过程的影响规律。结果表明:外源有机氮添加显著刺激了酸性森林土壤硝化活性,乙炔抑制实验表明自养氨氧化对酸性森林土壤硝化过程的贡献率90%。有机碳添加对土壤硝化活性未有显著影响,同时添加有机碳和无机铵态氮也未显著提高土壤硝化活性,而外源有机氮添加提高了土壤矿化速率并导致土壤NH3浓度升高,可能是土壤硝化活性、AOA和AOB数量显著增加的主要原因。
[Abstract]:In this paper, microcosmic laboratory culture experiments were carried out in subtropical acidic forest soils. The soil nitrification activity and the functional gene abundance of Ammonia-oxidizing ArchaeaAOAA, Ammonia-oxidizing bacterium AOBwere analyzed by adding organic carbon and organic nitrogen. The effects of exogenous organic carbon and organic nitrogen on the process of ammonia oxidation in acidic forest soil were studied. The results showed that the addition of exogenous organic nitrogen significantly stimulated the nitrification activity of acid forest soil, and the acetylene inhibition experiment showed that the contribution rate of autotrophic ammonia oxidation to the nitrification process of acid forest soil was 90%. The addition of organic carbon had no significant effect on the soil nitrification activity, and the addition of organic carbon and inorganic ammonium nitrogen did not significantly improve the soil nitrification activity, while exogenous organic nitrogen increased the mineralization rate of soil and led to the increase of soil NH3 concentration. It may be the main reason for the significant increase of AOA and AOB in soil nitrification activity.
【作者单位】：南京师范大学地理科学学院;江苏省物质循环与污染控制重点实验室;
【基金】：国家自然科学基金项目(41271255,41301260) 国家重点研发计划项目(2016YFD0200302)资助~~
【分类号】：S714

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