新肥菇原生地土壤有机碳氮测定条件优化
发布时间:2018-06-10 09:24
本文选题:土壤 + 新肥菇 ; 参考:《森林与环境学报》2017年03期
【摘要】:为优化新肥菇原生地土壤有机碳氮的测定条件,获取准确的碳氮含量,为该野生食用菌的栽培料营养配比提供科学依据,以采自新疆的新肥菇原生地土壤为试验材料,分别研究了浸提剂、浸提液浓度、样液比和浸提时间对土壤可溶性有机碳氮测定的影响。结果表明:浸提剂和浸提剂浓度对土壤可溶性有机碳的测定有重要影响,样液比和浸提时间对测定影响不显著。而浸提剂、浸提液浓度、样液比和浸提时间对土壤可溶性总氮的测定均无显著影响。选用新鲜土壤,在室温下以1.5 mol·L-1K2SO4为浸提剂,样液比1∶4,浸提时间20 min时测定结果重复性好,精确度高,符合检测要求。
[Abstract]:In order to optimize the determination conditions of organic carbon and nitrogen in the original soil of Pleurotus edodes, to obtain the accurate carbon and nitrogen content, and to provide scientific basis for the nutrient ratio of the wild edible fungus, the soil of the original soil of the new Pleurotus edodes collected from Xinjiang was used as the experimental material. The effects of extractant, concentration of extraction solution, ratio of sample to liquid and extraction time on the determination of soluble organic carbon nitrogen in soil were studied. The results showed that the concentration of extractant and extractant had an important effect on the determination of soil soluble organic carbon, but the ratio of sample to liquid and extraction time had no significant effect on the determination of soil soluble organic carbon. However, there were no significant effects on the determination of soluble total nitrogen (TN) in soil by extractant, concentration, ratio of sample to liquid and extraction time. When fresh soil was extracted at room temperature with 1.5 mol L ~ (-1) K _ (2SO _ 4) as extractant, the ratio of sample to liquid was 1: 4, and the extraction time was 20 min, the results were reproducible and accurate.
【作者单位】: 西南林业大学国家林业局西南地区生物多样性保育重点实验室;西南林业大学云南省高校森林灾害预警控制重点实验室;
【基金】:云南省林学一流学科建设项目(51600625) 中国科学院微生物研究所真菌学国家重点实验室开放基金项目
【分类号】:S153.6
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