大兴安岭火烧迹地土壤真菌群落结构及多样性研究

发布时间：2018-06-14 11:29

本文选题：大兴安岭 + 火烧迹地　；参考：《北方园艺》2017年10期

【摘要】：以大兴安岭火烧迹地在不同火烧年和不同火烧程度的土样为研究对象,揭示大兴安岭火烧迹地恢复过程中土壤真菌的多样性与群落变化特征,为火烧后森林生态系统的科学管理和森林更新提供理论依据。用PCR-DGGE法对土壤真菌进行了分析,采用Quantity one软件分析其多样性,通过序列的同源性比较构建系统发育树,真菌结构主成分分析采用Canoco等软件。结果表明:每个样品真菌种类丰富,不同样品有相同的真菌菌属也有不同的真菌菌属,17个优势条带测序所得菌属与黏滑菇属(Hebeloma)、蜡伞属(Hygrophorus)、蜡壳菌属(Sebacima)、管柄囊霉属(Funneliformis)亲缘关系最近,其中还有一些不可培养的真菌。不同火烧年和火烧程度之间土样真菌香农指数、丰富度、均匀度均达到显著差异(P0.05)。不同火烧程度、不同火烧年均能影响土壤真菌的群落结构。
[Abstract]:The diversity and community variation characteristics of soil fungi in the restoration process of the burning areas in Daxing'an Mountains were revealed by using soil samples from different years and different degrees of fire burning in Daxing'an Mountains as the research object. It provides theoretical basis for scientific management and forest regeneration of forest ecosystem after burning. The soil fungi were analyzed by PCR-DGGE, the diversity of fungi was analyzed by quantity one software, the phylogenetic tree was constructed by sequence homology comparison, and the principal component analysis of fungi structure was made by Canoco software. The results showed that each sample was rich in fungal species, and different samples had the same genus of fungi and different genera of fungi. The 17 dominant bands sequenced were closely related to the genus Hebelomae, Hygrophorusus, Sebacimaanus and Funneliformis. There are also some unculturable fungi. The Shannon index, richness and evenness of soil fungi reached significant difference between years and degrees of burning. The community structure of soil fungi can be affected by different fire degree and different annual fire.
【作者单位】：内蒙古师范大学生命科学与技术学院;
【基金】：内蒙古自治区科技计划资助项目(20130435)
【分类号】：S714.3

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