灰飞虱对噻嗪酮的抗性风险及机理

发布时间：2018-06-15 16:13

本文选题：灰飞虱 + 噻嗪酮　；参考：《应用生态学报》2016年01期

【摘要】：为研究灰飞虱对噻嗪酮的抗性发展规律及抗性生化机理,采用稻苗喷雾法对灰飞虱种群进行连续筛选获得高抗性品系,估算其现实遗传力并进行田间抗性风险预测;采用稻苗浸渍法测定杀虫剂对灰飞虱的毒力及交互抗性;利用生物化学方法测定不同品系之间的解毒酶活力,探讨灰飞虱对噻嗪酮的抗性生化机理.结果表明:用噻嗪酮对灰飞虱种群连续筛选32代,其抗性倍数达到168.49倍,现实遗传力h~2为0.11.当杀死率为80%~90%时,预计灰飞虱对噻嗪酮的抗性增长10倍,仅需要5~6代.田间实际的现实遗传力要比室内选择种群估计低一些,预计田间抗性提高10倍所需要时间会更长.交互抗性测定结果表明,灰飞虱抗噻嗪酮品系与吡虫啉和噻虫嗪之间有高水平交互抗性,与啶虫脒有低水平交互抗性,与吡蚜酮和毒死蜱无交互抗性.增效作用和解毒酶活力测定结果显示,抗性品系细胞色素P450单加氧酶活力提高最大,酯酶次之,谷胱甘肽-S-转移酶无显著变化.田间使用噻嗪酮防治灰飞虱存在较大抗性风险,可与吡蚜酮和毒死蜱等交替使用以延缓抗性发展;3种解毒酶中,细胞色素P450单加氧酶在灰飞虱对噻嗪酮的抗性发展中起到了重要作用.
[Abstract]:In order to study the development of resistance to thiazinone and the biochemical mechanism of resistance, rice seedling spray method was used to continuously screen the high resistant strains to estimate their realistic heritability and predict the resistance risk in the field. The virulence and cross resistance of insecticides to ash planthopper were determined by rice seedling impregnation method, and the biochemistry method was used to determine the detoxification enzyme activity among different strains to explore the biochemical mechanism of resistance to thiazinone. The results showed that using thiazinone to screen the population for 32 successive generations, the resistance ratio was 168.49 times and the realistic heritability was 0.11. When the killing rate was between 80 and 90, the resistance of planthopper to thiazinone was expected to increase tenfold, and only 5 to 6 generations were needed. The actual heritability of field was lower than that of indoor selection population, and it was estimated that it would take longer to increase field resistance by 10 times. The results of cross resistance test showed that there was high level of cross resistance with imidacloprid and thiamizine, low level cross resistance with acetamidine, and no cross resistance with pyrimidone and chlorpyrifos. The results of synergism and detoxification showed that cytochrome P450 monooxygenase activity was the highest in resistant strain, followed by esterase and glutathione -Stransferase. Field use of thiazinone to control ash planthopper had a high risk of resistance and could be alternately used with piriapyrone and chlorpyrifos in order to delay the development of resistance of three detoxifying enzymes. Cytochrome P450 monooxygenase plays an important role in the development of resistance of planthopper to thiazinone.
【作者单位】：山东农业大学植物保护学院;
【基金】：国家国际科技合作专项(2012DFG31740) 山东省现代农业产业技术体系玉米专项资助~~
【分类号】：S433

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