酰胺类除草剂对铜绿微囊藻的生长影响及氧化损伤效应

发布时间：2018-06-15 17:50

本文选题：酰胺类除草剂 + 铜绿微囊藻　；参考：《生态毒理学报》2016年01期

【摘要】：酰胺类除草剂的广泛使用对水生生态环境构成了潜在风险。为探究其对藻类的毒性作用,以铜绿微囊藻为对象,分别从藻类生长和氧化损伤效应角度探讨了甲草胺、乙草胺和丁草胺对铜绿微囊藻的毒性影响。实验结果显示,酰胺类除草剂对藻类的影响存在明显的滞后效应和剂量效应关系,低浓度暴露组刺激藻类增长,高浓度表现为抑制作用;3种酰胺类除草剂增加了铜绿微囊藻的氧化压力,并随着暴露时间的延长和浓度的增加而增强。其中,藻体内过氧化脂质降解产物丙二醛(MDA)含量明显增加,同时超氧化物歧化酶(SOD)和过氧化物酶(POD)活性也显著增强。经96 h暴露后,甲草胺(32.0 mg·L~(-1))、乙草胺(32.0 mg·L~(-1))和丁草胺(15.0 mg·L~(-1))暴露溶液中相对MDA含量分别为138%、204%和154%,相对SOD活性分别为116%、87%和115%,相对POD活性分别475%、278%和627%。结合生物量及氧化损伤效应实验结果可知,3种除草剂对铜绿微囊藻的毒性大小顺序为丁草胺乙草胺甲草胺。
[Abstract]:The widespread use of amide herbicides poses a potential risk to the aquatic ecological environment. The toxicity of alachlor, Acetochlor and butachlor to microcystis aeruginosa was studied from the point of view of algal growth and oxidative damage effects in order to study the toxicity of alachlor, Acetochlor and butachlor to microcystis aeruginosa. The results showed that there was a significant lag effect and dose-effect relationship between the effects of amide herbicides on algae, and the low concentration exposure group stimulated algae growth. High concentration showed inhibitory effect on the oxidation pressure of microcystis aeruginosa and increased with the increase of exposure time and concentration. Among them, the content of malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD) activity in algae increased significantly. After 96 h exposure, the relative MDA contents of alachlor (32.0 mg / L), Acetochlor (32.0 mg / L) and butachlor (15.0 mg / L) were 13844% and 1544%, respectively. The relative activity of SOD was 11687% and 1155.The relative activity of POD was 27.8% and 62728%, respectively. The results of biomass and oxidative damage showed that the toxicity of three herbicides to Microcystis aeruginosa was in the order of butachlor Acetochlor alachlor.
【作者单位】：浙江工业大学环境学院;浙江大学环境与资源学院;
【基金】：国家自然科学基金(21277126) 浙江省自然科学基金(LY15B070007) 教育部科技创新工程重大项目培育资金项目(708052)
【分类号】：X592

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