腺苷酸核糖基化因子BbarfA介导白僵菌孢子萌发和毒力

发布时间：2018-06-16 13:51

本文选题：腺苷酸核糖基化因子 + 球孢白僵菌　；参考：《微生物学报》2017年12期

【摘要】：【目的】探究腺苷酸糖基化因子ARF在球孢白僵菌(Beauveria bassiana)中存在种类及生物学功能。【方法】利用BLASTp搜索球孢白僵菌非冗余蛋白数据库,鉴定ARF并进行聚类分析,结合表达分析、反义抑制、超量表达野生型基因和GTP解离位点与结合位点突变的基因,解析其中1个ARF与白僵菌发育分化、逆境胁迫反应和毒力的关系。【结果】球孢白僵菌中存在至少6个ARF或类似蛋白,分别聚类于酵母、人类ARF及其类似蛋白的不同类群。其中BBA_01574与人类的ARF3、ARF4和ARF5聚为一类,命名为BbarfA。BbarfA在成熟的分生孢子和球形膨大时期表达明显高于芽管伸长期。反义抑制BbarfA加速了孢子萌发,提高了菌株毒力,而超量表达BbarfA和点突变GTP解离区域的BbarfA则延迟了孢子萌发速度,降低了菌株毒力。尽管BbarfA转录受高盐、氋渗、氧化和高温胁迫的诱导,但遗传修饰的转化子与野生菌株对上述胁迫反应的敏感性无明显差异。【结论】BbarfA介导分生孢子萌发和毒力。
[Abstract]:[objective] to explore the species and biological function of adenylate glycosylation factor ARF in Beauveria bassiana. [methods] the non-redundant protein database of Beauveria bassiana was searched by BLASTp, ARF was identified, cluster analysis and expression analysis were performed. Antisense inhibition, overexpression of wild-type gene and GTP dissociation site and binding site mutation gene, one of ARF and development differentiation of Beauveria bassiana were analyzed. The relationship between stress response and virulence. [results] there were at least 6 ARF or similar proteins in Beauveria bassiana, which were clustered in different groups of yeast, human ARF and similar proteins. BBA01574 and human ARF3 ARF4 and ARF5 were grouped into one group, named Bbarf A. BbarfA, the expression of BBA01574 in mature conidia and globular expansion stage was obviously higher than that in bud tube extension stage. Antisense inhibition of BbarfA accelerated spore germination and increased virulence, while overexpression of BbarfA and point mutant GTP dissociated BbarfA delayed spore germination and reduced virulence of the strain. Although BbarfA transcription was induced by high salt, osmotic, oxidative and high temperature stress, there was no significant difference in sensitivity between genetically modified transformants and wild strains. [conclusion] BbarfA mediates conidial germination and virulence.
【作者单位】：西南大学生物技术中心;西南大学植物保护学院;
【基金】：国家自然科学基金(30871668)~~
【分类号】：S476.12

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