氮高效转基因水稻OsNRT2.3b对土壤氨氧化细菌群落多样性的影响

发布时间：2018-06-16 20:22

本文选题：氮高效转基因水稻 + 氨氧化细菌　；参考：《农业环境科学学报》2017年06期

【摘要】：以氮高效转基因水稻Os NRT 2.3b两个不同株系N-04和N-08为研究对象,以非转基因亲本日本晴(Nipp)为对照,在田间小区试验条件下,设施氮和不施氮两种处理,采用变性梯度凝胶电泳(PCR-DGGE)技术,分析了氮高效转基因水稻在生长期对土壤氨氧化细菌群落多样性的影响。研究发现,水稻土壤氨氧化细菌丰富度指数在各生长期内品种间均不存在显著差异。两种处理条件下N-04的土壤氨氧化细菌香农-威纳指数仅在拔节期与Nipp有显著差异,其余生育期均无显著差异;在施氮条件下N-08的土壤氨氧化细菌香农-威纳指数在拔节期和抽穗扬花期与Nipp存在显著差异,不施氮条件下仅拔节期出现显著差异。两种处理条件下,N-04的土壤氨氧化细菌均匀度指数与Nipp相比整个生长期均无显著差异,而N-08在拔节期显著低于Nipp。测序结果表明,施氮和不施氮处理下氮高效转基因水稻(N-08和N-04)与Nipp相比土壤中拥有更多的亚硝化螺旋菌属(Nitrosospira)和亚硝化单胞菌属(Nitrosomonas)。研究表明,氮高效转基因水稻在个别生育期对香农-威纳指数和均匀度指数有显著差异,且其更有利于促进土壤铵态氮向硝态氮的转化。
[Abstract]:Two different lines, N-04 and N-08, of Os NRT 2.3b of high efficiency transgenic rice were studied with non transgenic parent Japan (Nipp) as control. Under the condition of field plot test, two treatments of nitrogen and no nitrogen were used, and the denatured gradient gel electrophoresis (PCR-DGGE) technique was used to analyze the soil ammonia in the growth period of nitrogen efficient transgenic rice. The study found that there was no significant difference between the varieties of the rice soil ammoxidation bacterial richness index in the growth period. The Shannon Weiner index of the N-04 soil ammoxidation bacteria was only significantly different from the Nipp at the jointing period under the two treatments, and there was no significant difference in the rest period of the growth period. The Shannon Weiner index of soil ammonia oxidizing bacteria in the lower N-08 was significantly different from Nipp at jointing stage and heading stage. There was significant difference in the jointing period under the condition of no nitrogen application. Under the two treatment conditions, there was no significant difference in the whole growth period of N-04's soil ammoxidation bacterial evenness index compared with Nipp, while N-08 was significantly lower than N at the jointing stage. The results of ipp. sequencing showed that more nitrogenous spirals (Nitrosospira) and nitrifying monomonas (Nitrosomonas) were found in the soil of high efficiency transgenic rice (N-08 and N-04) under nitrogen treatment and no nitrogen treatment. The study showed that the Shannon Weiner index and the evenness index of nitrogen efficient transgenic rice were obvious during the individual growth period. The difference is more conducive to the transformation of ammonium nitrogen to nitrate nitrogen in soil.
【作者单位】：农业部环境保护科研监测所农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室;东北农业大学资源与环境学院;
【基金】：国家自然科学基金项目(31301855) 转基因生物新品种培育重大专项(2016ZX08012005)~~
【分类号】：S154.3;S511

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