芸薹根肿菌单孢分离技术体系构建及沈阳地区根肿菌的鉴定

发布时间：2018-06-18 06:10

本文选题：十字花科作物 + 大白菜　；参考：《园艺学报》2017年12期

【摘要】：芸薹根肿菌严重危害大白菜和其他十字花科作物。由于根肿菌通常以多个生理小种混生状态进行侵染,导致抗病品种的抗性不能持久。以沈阳地区根肿菌为材料,进行了单孢分离技术体系构建,结果表明采取冷冻盒接菌法对两日龄大白菜幼苗进行单孢接种,可将侵染率提高到47.86%;采用基质过渡培养可将大白菜植株成活率提高到67%。利用上述根肿菌单孢分离技术体系对沈阳地区根肿菌进行单孢分离和Williams生理小种鉴定,结果表明沈阳地区根肿菌存在4号、8号、9号、11号生理小种,其中4号生理小种为优势小种。
[Abstract]:Bolting rhizomegaly seriously harms Chinese cabbage and other cruciferous crops. The resistance of resistant varieties can not be sustained due to the infection of rhizomegaly bacteria with multiple physiological races. The isolation technique system of single spore was constructed by using rhizomegaly bacteria in Shenyang area. The results showed that two day old Chinese cabbage seedlings were inoculated with single spores by freezing box inoculation method. The infection rate could be increased to 47.86, and the survival rate of Chinese cabbage could be increased to 67% by medium culture. The single sporulation isolation and Williams physiological race identification of rhizomegaly bacteria in Shenyang area were carried out by using the technique system mentioned above. The results showed that there were 4, 8, 9 and 11 physiological races of rhizomegaly bacteria in Shenyang area. Physiological race No. 4 was dominant.
【作者单位】：沈阳农业大学园艺学院辽宁省十字花科蔬菜遗传育种重点实验室;
【基金】：国家现代农业产业技术体系建设专项资金项目(CARS-25-A-03) 辽宁省自然科学基金项目(2013020071)
【分类号】：S432.4

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