辣椒疫病罹病植株根际土壤细菌群落多样性分析

发布时间：2018-06-21 00:36

本文选题：辣椒疫病 + 根际土壤　；参考：《南方农业学报》2017年06期

【摘要】：【目的】分析辣椒疫病罹病植株根际土壤细菌群落多样性,为辣椒疫病防控提供理论依据。【方法】采集湖南长沙、吉首及永州地区辣椒疫病罹病植株根际土壤,运用IlluminaMiSeq高通量测序技术对土壤样品基因组DNA的16S rDNA-V4区进行测序分析,明确不同地区罹病辣椒植株根际土壤细菌群落多样性。【结果】3个地区罹病辣椒根际土壤的细菌OTU数量存在差异,独有OTU占总体比例各有差异,其中永州地区占其总数的30.18%、吉首地区占其总数的41.83%、长沙地区占其总数的24.07%,3个地区共有OTU占所有检测到OTU的14.95%。α多样性指标均表现为长沙地区最低,吉首地区最高。在门水平上,3个地区的罹病辣椒根际土壤微生物组成相似,优势菌门为Acidobacteria(酸杆菌门)、Proteobacteria(变形菌门)、Actinobacteria(放线菌门)和Chloroflexi(绿弯菌门),但3个地区在相对丰度上表现一定差异。【结论】湖南永州、吉首和长沙地区辣椒疫病罹病植株根际土壤细菌多样性存在差异,以吉首最高、长沙最低;3个地区的罹病辣椒根际土壤微生物组成相似,但相对丰度存在差异。
[Abstract]:[objective] to analyze the diversity of bacterial communities in the rhizosphere of pepper blight plants, and to provide a theoretical basis for the prevention and control of pepper blight. [methods] the rhizosphere soil of pepper blight plants in Changsha, Jishou and Yongzhou, Hunan Province, was collected. The 16s rDNA-V4 region of genomic DNA of soil samples was sequenced by IlluminaMiSeq high-throughput sequencing technique. The diversity of bacterial communities in rhizosphere soil of diseased pepper plants was determined. [results] there were differences in the number of bacterial OTU in the soil of infected pepper rhizosphere in three regions, and the proportion of OTU in the soil was different. Yongzhou accounted for 30.18 percent of the total, Jishou accounted for 41.83 percent of the total, Changsha accounted for 24.07 percent of the total. The three regions had OTU accounted for all the detected OTU 14.95a diversity indicators were the lowest in Changsha and the highest in Jishou. At the gate level, the soil microbes in the rhizosphere of diseased pepper were similar in three regions. The dominant bacteria were Acidobacteria (Proteobacteria) and Chloroflexia (Actinobacteria). The diversity of bacteria in rhizosphere soil of Phytophthora infestans in Jishou and Changsha was different, the highest in Jishou and the lowest in Changsha, and the composition of microorganism in rhizosphere of infected pepper was similar, but the relative abundance was different.
【作者单位】：永州市农业科学研究所;湖南省农业科学院植物保护研究所/园艺作物病虫害治理湖南省重点实验室;湖南农业大学生物科学技术学院;广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室;
【基金】：国家自然科学基金项目(31501696) 国家高层次人才特殊支持计划项目(2016RS2019) 广西作物病虫害生物学重点实验室基金项目(14-045-50-KF-4)
【分类号】：S154.3;S436.418

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