溶磷细菌对复垦土壤有效磷及各形态无机磷含量的影响

发布时间：2018-06-24 23:14

本文选题：溶磷细菌 + 复垦土壤　；参考：《河南农业科学》2017年09期

【摘要】：为探索溶磷细菌在复垦土壤中对磷的作用效果,采用室内摇瓶培养的方法,研究了溶磷细菌及其组合对磷酸钙的溶解能力,从而确定最佳组合,并探讨最佳组合溶磷细菌对复垦土壤有效磷及各形态无机磷含量的影响。结果表明,拉恩式菌(W2)+荧光假单胞菌1(W3)+荧光假单胞菌2(W4)培养液的有效磷含量最高,为609.1 mg/L,其为最佳溶磷细菌组合。施用此溶磷细菌组合肥可以增加复垦土壤有效磷含量,复垦土壤有效磷含量表现为BG(溶磷细菌肥+磷酸钙)处理B(溶磷细菌肥)处理MG(基质+磷酸钙)处理M(基质)处理CK(空白),其中,B处理在苗期、拔节期、收获期分别比M处理显著增加15.7%、119.7%、54.1%,BG处理在苗期、拔节期、收获期分别比MG处理显著增加55.8%、91.8%、88.9%。在玉米苗期和收获期,施用溶磷细菌可以增加复垦土壤Ca_2-P、Ca_8-P、Fe-P、Al-P含量,其中收获期B处理比基质处理M分别显著增加86.6%、88.7%、38.6%、83.3%;复垦土壤O-P和Ca_(10)-P含量均表现为MGBGMBCK,溶磷细菌对复垦土壤O-P含量影响较小,对Ca_(10)-P含量影响较大,苗期和收获期B、BG处理Ca_(10)-P含量分别比对应的基质处理M、MG处理降低5.8%、23.1%和9.5%、24.4%,即溶磷细菌可以减少复垦土壤Ca_(10)-P的含量。土壤有效磷含量与土壤Ca_2-P、Ca_8-P、Al-P、Fe-P含量呈极显著正相关,与O-P、Ca_(10)-P含量无显著相关性。
[Abstract]:In order to explore the effect of phosphorus dissolving bacteria on phosphorus in reclaimed soil, the dissolving ability of phosphorus dissolving bacteria and their combinations to calcium phosphate was studied by the method of shaking flask culture in laboratory, so as to determine the best combination. The effects of optimum combination of phosphorus soluble bacteria on available phosphorus and inorganic phosphorus contents in reclaimed soil were investigated. The results showed that the highest available phosphorus content was 609.1 mg / L in the culture medium of Pseudomonas fluorescein 1 (W3) and Pseudomonas fluorescein (W4), which was the best combination of phosphorus solubilizing bacteria. The available phosphorus content of reclaimed soil could be increased by applying this phosphate solubilizing bacteria group. The available phosphorus content of reclaimed soil was BG (phosphate soluble bacterial fertilizer calcium phosphate) treatment B (phosphate soluble bacterial fertilizer) treatment MG (matrix calcium phosphate) treatment M (matrix calcium phosphate) treatment CK (blank), in which B treatment was at seedling stage and jointing stage. The harvest period was significantly higher than that of M treatment by 15.7and 119.7and 54.1BG at seedling stage, jointing stage and harvest stage, respectively, and increased 55.8% and 91.8%, respectively, compared with MG treatment in the seedling stage, jointing stage and harvest stage, respectively. At the seedling stage and harvest stage of maize, the application of phosphorus soluble bacteria could increase the content of Ca2-Pna-Ca8-PnFe-PnAl-P in the reclaimed soil. The contents of O-P and Ca _ (10) -P in reclaimed soil showed MGBGMBCK. the phosphorus dissolving bacteria had little effect on O-P content in reclaimed soil, but had a greater effect on Ca10 -P content in reclaimed soil than that in substrate treatment M, which increased 86.6% and 38.6%, respectively, compared with that of substrate treatment M, and the contents of O-P and Ca10 -P in reclaimed soil showed MGBGMBCK. the effect of phosphorus dissolving bacteria on O-P content in reclaimed soil was less than that in substrate treatment. The contents of Ca _ (10) -P of BBG treatment at seedling stage and harvest stage were lower than those of MMG treatment by 5.8% and 9.5%, respectively. That is, phosphorus soluble bacteria could reduce the content of Ca _ (10) -P in reclaimed soil. The content of available phosphorus in the soil was positively correlated with the content of Ca2- P0, Ca8-P0, Al-P0, Fe-P, but not with the content of Ca10-P in the soil.
【作者单位】：安顺学院资源环境与工程学院;山西农业大学资源环境学院;
【基金】：贵州省科技合作计划项目(黔科合LH字[2016-7280号]);贵州省科技合作计划项目(黔科合LH字[2014-7515号]) 山西省科技攻关项目(20121101009-4) 安顺学院博士科研启动基金项目(asubsjj201601号)
【分类号】：S153.6

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