典型农田黑土细菌与土壤碳氮相关性研究

发布时间：2018-06-29 07:41

本文选题：有机质含量 + 土壤C、N　；参考：《黑龙江大学》2015年硕士论文

【摘要】：本研究以黑土生产力土壤空间移位长期定位试验为平台,于2013-2014年种植玉米,研究5种不同土壤有机质含量农田黑土-黑龙江北安(SOM11)、黑龙江嫩江(SOM6)、黑龙江海伦(SOM5)、吉林德惠(SOM3)、吉林梨树(SOM1.7)功能性细菌的变化规律及其与土壤碳、氮等养分的关系,结果表明:5种不同有机质含量农田黑土生理功能细菌变化规律为:细菌占微生物总数的80%左右,其总量随土壤有机质含量的增加而增加。氨化细菌数量变幅在1.92~2.81×104/个g-1之间,硝化细菌数量变幅在0.92~2.74104/个g-1之间,反硝化细菌数量变幅在0.59~2.03104/个g-1之间,三者随土壤有机质含量的增加而增加;亚硝化细菌数量的变幅在0.59~3.17104/个g-1之间,亚硝化细菌变化规律随着有机质含量的降低而增加。5种不同有机质含量农田黑土土壤碳氮养分的变化规律为:土壤铵态氮、有效态氮、固定态铵、微生物量氮、土壤总有机氮含量均是随作物生育期的延长而降低,铵态氮的变化在9.18~36.81mg/区之间,有效态氮变幅在41.82~87.43mg/区之间,固定态铵的变幅在5.86~27.01mg/区之间,微生物量氮的变幅在3.21~19.55mg/区之间,总有机氮的变幅在5.55~22.90mg/区之间;硝态氮的变化规律随生育期的延长而增加,硝态氮含量的变幅在13.49~62.18 mg/区之间。利用SAS软件,通过相关分析与通径分析,结果显示土壤有机碳、全氮,铵态氮、硝态氮、固定态铵、微生物量氮与氨化细菌、硝化细菌、反硝化细菌都存在显著或极显著的相关关系,对土壤全氮直接作用大小顺序:氨化细菌(0.456)、硝化细菌(0.274)亚硝化细菌(0.139)、反硝化细菌(0.109);对有机质直接作用顺序氨化细菌(0.334)、反硝化细菌(0.180)、硝化细菌(0.160)亚硝化细菌(0.078);对有机氮、硝态氮硝化细菌直接作用较大(0.025;0.499),对固定态铵、微生物量碳氮直接作用是氨化细菌(0.258;0.661;0.561)较大。
[Abstract]:In this study, maize was planted in 2013-2014 on the platform of long-term spatial displacement experiment of black soil productivity soil. To study the changes of five kinds of soil organic matter content in farmland black soil-Heilongjiang Bei'an (SOM11), Heilongjiang Nenjiang (SOM6), Heilongjiang Helen (SOM5), Jilin Dehui (SOM3), Jilin pear tree (SOM1.7) and their relationship with soil carbon, nitrogen and other nutrients. The results showed that the changes of physiological function bacteria of five different organic matter contents in black soil of farmland were about 80% of the total number of microbes, and the total amount of bacteria increased with the increase of soil organic matter content. The number of ammoniated bacteria varied from 1.92 to 2.81 脳 10 ~ (4) / g ~ (-1), the number of nitrifying bacteria varied from 0.92 to 2.74104 / g ~ (-1), and the number of denitrifying bacteria varied from 0.590.03104 / g ~ (-1) to 0.592.03104 / g ~ (-1), which increased with the increase of soil organic matter content. The number of nitrifying bacteria varied from 0.59 to 3.17104 / g ~ (-1). With the decrease of organic matter content, the changes of soil carbon and nitrogen nutrients were as follows: soil ammonium nitrogen and available nitrogen. The content of fixed ammonium, microbial biomass nitrogen and soil total organic nitrogen decreased with the extension of crop growth period. The change of ammonium nitrogen was between 9.18~36.81mg/ region, the range of available nitrogen was between 41.82~87.43mg/ region, the range of fixed ammonium change was between 5.86~27.01mg/ region. The variation of microbial biomass nitrogen was between the 3.21~19.55mg/ region and the total organic nitrogen region between the 5.55~22.90mg/ regions, and the variation of nitrate nitrogen increased with the extension of the growth period, and the variation of nitrate content was between 13.49 ~ 62.18 mg/. Using SAS software, correlation analysis and path analysis, the results showed that soil organic carbon, total nitrogen, ammonium nitrogen, nitrate nitrogen, fixed ammonium, microbial biomass nitrogen and ammoniated bacteria, nitrifying bacteria, Denitrifying bacteria have significant or extremely significant correlation, Order of direct action on soil total nitrogen: ammonifying bacteria (0.456), nitrifying bacteria (0.274) nitrifying bacteria (0.139), denitrifying bacteria (0.109), direct action on organic matter sequence ammonifying bacteria (0.334), denitrifying bacteria (0.180), nitrifying bacteria (0.160) nitrifying bacteria (0.078), and organic nitrogen, The direct action of nitrate nitrifying bacteria was greater (0.025 ~ 0.499). For fixed ammonium, the direct action of microbial biomass carbon and nitrogen was ammoniated bacteria (0.2588 ~ 0.661n ~ (0.561).
【学位授予单位】：黑龙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S154.3

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