宁夏产甘草内生细菌分离纯化、拮抗植物病原菌菌株的筛选及鉴定

发布时间：2018-06-30 01:35

本文选题：甘草 + 内生菌　；参考：《时珍国医国药》2017年09期

【摘要】：目的对宁夏产甘草根茎叶中内生细菌分离纯化,以明确甘草中内生细菌的多样性及分布规律;初步筛选出对病原菌有拮抗效果的细菌作为优势菌种,进一步对其进行鉴定,为甘草内生细菌资源的进一步开发提供科学依据。方法采用组织块法,在牛肉膏蛋白胨培养基中分离纯化内生细菌;采用滤纸片法,观察有无抑菌圈及抑菌圈的大小来检测内生细菌对病原菌的拮抗效果;采用16Sr DNA测序分析,对比核糖体数据库鉴定典型菌种。结果从甘草中分离出内生细菌100株,其中根占76%、茎占15%、叶占9%;初步筛选出对西瓜枯萎病菌、小麦根腐病菌、黄花枯萎病菌、烟草赤星病菌、棉花枯萎病菌等病原菌综合抑制效果较好的细菌菌种8种(G2、G3、G5、G8、G9、G15、Y1和Y2),进一步鉴定这8种细菌发现G2为地衣芽孢杆菌,G3、G8为蜡样芽胞杆菌,G5、G9、Y1为短小芽胞杆菌。结论宁夏产甘草中含有丰富的内生细菌,且以根中居多;其中能够对多种病原菌有拮抗效果菌种为芽孢杆菌菌属。
[Abstract]:Objective to isolate and purify endophytic bacteria from stems and leaves of Glycyrrhiza uralensis in Ningxia, to clarify the diversity and distribution of endophytic bacteria in Glycyrrhiza uralensis, and to screen the bacteria that have antagonistic effect on pathogenic bacteria as dominant strains, and to further identify them. It provides scientific basis for the further development of endophytic bacteria resources of Glycyrrhiza uralensis. Methods endophytic bacteria were isolated and purified from beef extract peptone medium by tissue block method, and the antagonistic effect of endophytic bacteria against pathogenic bacteria was detected by using filter paper method to detect the antagonistic effect of endophytic bacteria against pathogenic bacteria, and 16Sr DNA sequencing was used to detect the antagonistic effect of endophytic bacteria against pathogenic bacteria. Compare ribosomal database to identify typical strains. Results 100 strains of endophytic bacteria were isolated from Glycyrrhiza uralensis, of which 76 were root, 15 were stem, and 9 were leaf. Eight strains of cotton wilt bacteria (G2G3G5, G8, G9, G15, Y1 and Y2) were identified as Bacillus licheniformis G3, G8 as Bacillus cereus G5G9Y1, and Bacillus pumilus G5G9Y1 as Bacillus cereus G5G9Y1, which were identified as Bacillus licheniformis, Bacillus cereus, and Bacillus cereus G5G9Y1. Conclusion there are abundant endophytic bacteria in Glycyrrhiza uralensis in Ningxia, and the most endophytic bacteria are in root.
【作者单位】：宁夏医科大学实验动物中心;宁夏医科大学药学院;宁夏回药现代化工程技术研究中心宁夏回医药协同创新中心宁夏医科大学回医药现代化省部共建教育部重点实验室;
【基金】：国家自然科学基金(No.31260304;No.31460330)
【分类号】：S476.1;S567.71

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