塔吉克斯坦哈特隆州土壤可培养细菌多样性分析

发布时间：2018-07-04 22:24

本文选题：塔吉克斯坦 + 纯培养　；参考：《干旱区地理》2016年01期

【摘要】：采用纯培养、结合核糖体DNA扩增片段限制性酶切分析(16S r DNA PCR-RFLP)、RepPCR及16S r DNA序列测定和系统发育分析方法研究塔吉克斯坦哈特隆州土壤可培养细菌群落结构和种群多样性。从哈特隆州的农田、天然草地、林地及荒漠土壤中分离获得110个菌株,分布在厚壁菌门(Firmicutes)(80.9%)、变形杆菌门(Proteobacteria)(17.2%)和放线菌门(Actinobacteria)(1.85%)三个类群中,归属于芽孢杆菌(Bacillus)、寡养单胞菌(Stenotrophomonas)、短杆菌(Brevibacterium)、节杆菌(Arthrobacter)、棍状杆菌(Clavibacter)、短波单胞菌(Brevundimonas)、贪食菌(Variovorax)、假单胞杆菌(Pseudomonas)、芽孢八叠球菌(Sporosarcina)、赖氨酸芽孢杆菌(Lysinibacillus)、无色杆菌属(Achromobacter)和Rheinheimera共12个属中。其中Bacillus(71.8%)为该地区土壤可培养细菌绝对优势种群,Pseudomonas(10.9%)为次优势种群。对优势种群Bacillus的Rep-PCR基因组指纹图分析表明,63株分离物有17种不同的多态性指纹图谱,具有较为丰富的多样性特征。不同样地土壤中可培养细菌的种群分布、结构组成及多样性存在明显差异,与土壤环境密切相关。Shannon多样性指数、Simpson优势度和Pielou均匀度指数与土壤有机质、全氮、碱解氮及含水量呈显著正相关(p0.05)。土壤速效钾含量也是显著影响细菌Shannon多样性、Simpson优势度的重要因素。研究不仅为后期功能菌株的筛选提供了基础,也为塔国不同土地利用方式下土壤环境评价提供了依据。
[Abstract]:The community structure and population diversity of culturable bacteria in the soil of Khatlon, Tajikistan, were studied by pure culture, restriction enzyme digestion analysis (16s r DNA PCR-RFLP) and sequencing of 16s r DNA sequence and phylogenetic analysis. 110 strains were isolated from farmland, natural grassland, woodland and desert soil in Hatteron. They were found in three groups: Firmicutes (80.9%), Proteobacteria (17.2%) and actinomycetes (1.85%). It belongs to Bacillus, Stenotrophomonas, Brevibacterium, Clavibacter, Brevundimonas, Variovorax, Pseudomonas, Sporosarcina, Lysinibacillus, (Achromobacter) and Rheimera. Among them, Bacillus (71.8%) is the subdominant population of soil culturable bacteria, Pseudomonas (10.9%). The genomic fingerprinting analysis of Bacillus showed that there were 17 different polymorphic fingerprints of the isolates of Bacillus, which had rich diversity characteristics. The population distribution, structural composition and diversity of culturable bacteria in different soils were significantly different. The Shannon diversity index Simpson dominance and Pielou evenness index were closely related to soil environment and soil organic matter and total nitrogen. There was a significant positive correlation between alkali hydrolyzed nitrogen and water content (p0.05). Soil available potassium content was also an important factor that significantly affected the dominance of Shannon diversity Simpson bacteria. The study not only provided the basis for screening of functional strains in the later stage, but also provided the basis for soil environmental assessment under different land use patterns in Tajikistan.
【作者单位】：新疆农业大学农学院;新疆自治区高校农林有害生物监测与安全防控重点实验室;塔吉克斯坦农业大学;
【基金】：国家国际科技合作计划资助(2010DFA92720-13) 溢达奖学金资助
【分类号】：S154.3

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