花生根际多功能高效促生菌的筛选鉴定及其效应研究

发布时间：2018-07-24 09:00

【摘要】：为了获得具有固氮、解磷解钾及合成生长素的多功能花生根际促生菌(PGPR),提高和改善河南花生种植区的花生产量和品质。从华北地区花生根际砂质潮土中筛选出5株多功能促生菌HS4、HS7、HS9、HS10、HS11,通过对其各功能指标的对比挑选出菌株HS9,其固氮酶活性、解磷解钾以及产IAA能力分别达到15.53 nmol C2H4/h·m L、279.23 mg/L、22.5 mg/L和40.96 mg/L。经形态观察、生理生化指标以及16S r DNA保守序列鉴定,确定该菌株为弯曲芽胞杆菌(Bacillus flexus)。最后通过花生盆栽实验验证其促生能力。盆栽实验表明:接种HS9能显著提高土壤中速效磷、钾含量,极显著地提高了土壤中IAA的含量;植株的根总长、根表面积、根体积和根尖数分别增加了109.60%、84.30%、76.08%和386.24%,促进植株对养分的吸收和利用,提高植株生物量以及养分含量;植株鲜重、株高、叶绿素值(Soil and plant analyzer development,SPAD)及氮、磷、钾含量分别提高了70.05%、28.35%、16.06%、23.11%、83.04%和23.95%。该多功能菌株对花生具有良好的促生作用,具有广阔的农业应用前景。
[Abstract]:In order to obtain the multifunctional peanut rhizosphere growth-promoting bacteria (PGPR),) with nitrogen-fixing, phosphorolytic potassium and synthetic auxin, the yield and quality of peanut in Henan Province were improved. Five multifunctional growth-promoting bacteria HS4 HS7HS9 HS10 HS11 were screened from sandy aquic soil of peanut rhizosphere in North China. The nitrogenase activity, phosphorolytic potassium and IAA production of HS9 strain were up to 15.53 nmol C2H4/h / L ~ 279.23 mg / L ~ 22.5 mg/L and 40.96 mg 路L ~ (-1) 路L ~ (-1), respectively. The strain was identified as Bacillus campylobacter (Bacillus flexus). By morphological observation, physiological and biochemical indexes and 16s r DNA conserved sequence identification. Finally, the growth promoting ability of peanut was verified by pot experiment. Pot experiment showed that inoculation with HS9 could significantly increase the content of available phosphorus and potassium in soil, increase the content of IAA in soil, and increase the total root length and root surface area of plant. The root volume and the number of root tips increased by 109.60% and 386.24%, respectively, to promote the absorption and utilization of nutrients, to increase the biomass and nutrient content of the plants, to increase the plant fresh weight, plant height, chlorophyll value (Soil and plant analyzer development Spad) and the contents of nitrogen, phosphorus and potassium by 70.0528.3516.06% 23.1183.04% and 23.9595%, respectively. The multifunctional strain has a good promoting effect on peanut and has a broad prospect of agricultural application.
【作者单位】：河南农业大学资源与环境学院;山东省土壤肥料总站;
【基金】：国家自然科学基金项目(41401274) 河南省科技攻关计划(国际科技合作)项目(162102410031) 河南省教育厅项目(15A210012,14B210025)
【分类号】：S154.3;S565.2

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