白芨内生细菌组成及多样性分析

发布时间：2018-08-20 14:37

【摘要】：【目的】调查白芨内生细菌种类及多样性,为更好地保护和开发利用白芨资源提供科学依据。【方法】应用Illumina Mi Seq高通量测序技术测定白芨内生细菌的16S r DNA-V4变异区序列,应用Qiime和Mothur等软件整理和统计样品序列数目和操作分类单元(OTUs)数量,分析物种丰度及分布的差异。【结果】获得用于分析的有效序列和OTU数为49550/48;稀释曲线表明测序深度充分,OTU的数量接近于饱和。白芨内生细菌主要分布于鞘脂单胞菌属(Sphingomonas,32.26%)、地杆菌属(Pedobacter,16.13%)、盐单胞菌属(Halomonas,16.13%)、土壤杆菌属(Agrobacterium,12.90%)、Kaistobacter属(6.45%)、希瓦氏菌属(Shewanella,6.45%)、假单胞菌属(Pseudomonas,6.45%)和短波单胞菌属(Brevundimonas,3.23%)8个属。【结论】鞘脂单胞菌属、地杆菌属、盐单胞菌属和土壤杆菌属是白芨内生细菌的优势种群。Illumina Mi Seq高通量测序技术能准确反映植物内生微生物的种类组成和真实比例,在植物内生微生物研究中具有明显的优势和可行性。
[Abstract]:[objective] to investigate the species and diversity of endophytic bacteria in Bletilla striata so as to provide a scientific basis for better protection and utilization of Bletilla striata resources. [methods] the 16s r DNA-V4 mutation region of endophytic bacteria in Bletilla striata was sequenced by Illumina Mi Seq high-throughput sequencing. Using software such as Qiime and Mothur to sort out and count the number of sample sequences and the number of operation classification units (OTUs), Analysis of species abundance and distribution. [results] the effective sequence and OTU number for analysis were 49550 / 48.The dilution curve showed that the number of OTU with sufficient sequencing depth was close to saturation. Bletilla striata endophytic bacteria are mainly distributed in 32.26% of Sphingomonas, 16.13% of Pedobacteromonas, 16.13% of Halomonas, 12.90% of Agrobacterium, 6.45% of Kaistobacter, 6.45% of Shewanellaa, 6.45% of Pseudomonas and 3.23% of Brevundimonasmonas. [conclusion] Halomonas and Agrobacterium are the dominant species of endophytic bacteria in Bletilla striata. Illumina Mi Seq high throughput sequencing technique can accurately reflect the species composition and true proportion of endophytic microbes in plants. It has obvious advantages and feasibility in the study of plant endophytic microorganisms.
【作者单位】：昆明学院农学院;云南省都市特色农业工程技术研究中心;中国科学院昆明动物研究所;昆明学院生命科学与技术系;
【基金】：国家自然科学基金项目(31460491) 云南省教育厅科学研究项目(2014Y390) 昆明学院人才引进项目(YJL14005) 云南省高校优势特色重点学科(生态学)建设项目(05000511311)
【分类号】：S567.239;S182

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