解淀粉芽孢杆菌L-H15固态发酵参数优化与试验

发布时间：2018-08-21 09:49

【摘要】：解淀粉芽孢杆菌是一种植物根际促生细菌。通过响应面试验和正交试验,对分离筛选得到的解淀粉芽孢杆菌L-H15的最大活菌数进行了优化,确定了发酵工艺最佳参数。在黄瓜育苗期施用高活性固态菌剂,进行实际效果试验验证。结果表明,在优化的发酵条件下,即由1∶2.8的麦麸和豆粕组成固态基质,外加20%的稻谷壳,0.2%乳糖,0.38%大豆蛋白胨,并用0.4 mL/g的液料比混合均匀;装瓶量46%,解淀粉芽孢杆菌L-H15活菌数高达1.68×10~(10)CFU/g;L-H15固态发酵菌剂能明显促进黄瓜幼苗的茎粗、株高、叶面积及生物量的累积(P0.05),根系活力增强,矿质元素含量也有显著性提高。因此,解淀粉芽孢杆菌L-H15能够有效促进植株生长,固态菌剂的研发为减少农药化肥的施用提供了技术支撑,是一种提高农作物产量的新型方法。
[Abstract]:Bacillus amylolyticus is a rhizosphere-promoting bacteria. By means of response surface test and orthogonal test, the maximum viable number of L-H15 isolated from Bacillus amylolyticus was optimized, and the optimum fermentation parameters were determined. The application of high active solid bacteria agent in cucumber seedling stage was tested and verified. The results showed that under the optimum fermentation conditions, the solid substrate was composed of 1: 2.8 wheat bran and soybean meal, and 20% rice husk was added with 0.2% lactose and 0.38% soybean peptone, and the mixture was evenly mixed with 0.4 mL/g. The number of viable bacteria of Bacillus amylolyticus L-H15 was 1.68 脳 10 ~ (10) CFU / g ~ (10) CFU / GG ~ (L-H15) solid state fermentation agent, which could significantly promote the accumulation of stem diameter, plant height, leaf area and biomass of cucumber seedlings (P0.05), increase the activity of root system and increase the content of mineral elements. Therefore, Bacillus amylolyticus L-H15 can promote plant growth effectively. The research and development of solid bacteria provide technical support for reducing the application of pesticides and fertilizers, and it is a new method to increase crop yield.
【作者单位】：中国农业大学食品科学与营养工程学院;中国农业科学院蔬菜花卉研究所;
【基金】：国家自然科学基金项目(31172001) 公益性行业(农业)科研专项(201303014)
【分类号】：S182

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