新蚜虫疠霉菌丝和孢子阶段的转录组学分析
发布时间：2018-08-30 19:16
【摘要】：新蚜虫疠霉(Pandora neoaphidis)是虫霉门(Entomophthoromycota)中极具代表性的蚜虫专化性病原真菌,分布广泛,可侵染多种蚜虫。适宜条件下可在较短的时期内形成流行病,在蚜虫自然防治中起主导作用。为了系统分析了两个阶段的基因表达和功能相关的转录组信息,本研究通过二代测序平台Illumina Hiseq 2500对新蚜虫疠霉的两种发育形态(菌丝和分生孢子)进行转录组测序。得到90 639个不含N(模糊碱基)的组装片段(contigs)和49 138条单基因簇(unigenes)。49 138条单基因簇经基因本体论(Gene Ontology,GO)功能显著性富集分类后归到47种功能分支中,相关的基因功能主要归类至分子功能、细胞组分和生化过程三大类中。根据每百万reads中来自于某基因每千碱基长度的reads数(reads per kilobase per million of mapped reads,RPKM)的表达量算法一共得到3 555个差异表达的基因,其中上调的基因有2 294个,下调的基因有1 261个。利用京都基因及基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)对差异表达基因进行途径显著性富集分析后发现与之相关的代谢路径有22条,其中糖胺聚糖降解和类胡萝卜素生物合成两条途径显著上调。本研究为进一步挖掘和分析与新蚜虫疠霉生长发育相关的功能基因提供了理论依据。
[Abstract]:(Pandora neoaphidis) is a representative aphids specific to venereal fungi in (Entomophthoromycota). It is widely distributed and can infect many kinds of aphids. Under suitable conditions, epidemic diseases can be formed in a short period of time and play a leading role in the natural control of aphids. In order to systematically analyze the transcriptional information related to gene expression and function in two stages, the second generation sequencing platform Illumina Hiseq 2500 was used to sequence the two developmental forms (hyphae and conidium) of Pandora neoaphids. 90,639 (contigs) fragments without N (fuzzy base) and 49,138 single gene clusters (unigenes). 49,138 single gene clusters were classified into 47 functional branches by functional enrichment and classification of Gene Ontology,GO. The related gene functions are classified into three categories: molecular function, cellular component and biochemical process. A total of 3 555 differentially expressed genes were obtained according to the algorithm of reads number (reads per kilobase per million of mapped reads,RPKM per million reads, in which 2 294 genes were up-regulated and 1 261 genes were down-regulated. Significant enrichment of differentially expressed genes by using Kyoto gene and genome encyclopedia (Kyoto Encyclopedia of Genes and Genomes,KEGG showed that there were 22 related metabolic pathways. Glycosaminoglycan degradation and carotenoid biosynthesis were significantly up-regulated. This study provides a theoretical basis for further research and analysis of functional genes related to the growth and development of Pandora neoaphids.
【作者单位】： 中国计量大学生命科学学院/浙江省生物计量与检验检疫重点实验室;宜宾学院生命科学与食品工程学院;
【基金】：国家自然科学基金(No.31461143030) 浙江省自然科学基金杰出青年项目(No.LR12C03001) 福建省泉州市科技计划项目(No.2015Z52)
【分类号】：S476.12
本文编号：2214037
[Abstract]:(Pandora neoaphidis) is a representative aphids specific to venereal fungi in (Entomophthoromycota). It is widely distributed and can infect many kinds of aphids. Under suitable conditions, epidemic diseases can be formed in a short period of time and play a leading role in the natural control of aphids. In order to systematically analyze the transcriptional information related to gene expression and function in two stages, the second generation sequencing platform Illumina Hiseq 2500 was used to sequence the two developmental forms (hyphae and conidium) of Pandora neoaphids. 90,639 (contigs) fragments without N (fuzzy base) and 49,138 single gene clusters (unigenes). 49,138 single gene clusters were classified into 47 functional branches by functional enrichment and classification of Gene Ontology,GO. The related gene functions are classified into three categories: molecular function, cellular component and biochemical process. A total of 3 555 differentially expressed genes were obtained according to the algorithm of reads number (reads per kilobase per million of mapped reads,RPKM per million reads, in which 2 294 genes were up-regulated and 1 261 genes were down-regulated. Significant enrichment of differentially expressed genes by using Kyoto gene and genome encyclopedia (Kyoto Encyclopedia of Genes and Genomes,KEGG showed that there were 22 related metabolic pathways. Glycosaminoglycan degradation and carotenoid biosynthesis were significantly up-regulated. This study provides a theoretical basis for further research and analysis of functional genes related to the growth and development of Pandora neoaphids.
【作者单位】： 中国计量大学生命科学学院/浙江省生物计量与检验检疫重点实验室;宜宾学院生命科学与食品工程学院;
【基金】：国家自然科学基金(No.31461143030) 浙江省自然科学基金杰出青年项目(No.LR12C03001) 福建省泉州市科技计划项目(No.2015Z52)
【分类号】：S476.12
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