基于正交试验设计的土壤种子库萌发影响因素优化研究

发布时间：2018-09-05 06:43

【摘要】：土壤种子库(Soil Seed Bank)是土壤及土壤表面落叶层中所有具有生命力的种子的总和,是植被天然更新的种源贮备库。选取表土铺设厚度、覆盖物厚度、引入乔木种子数量3个因素设置正交试验,分析正交试验后土壤种子库的特征,采用极差分析法、主成分分析法、指标体系法对不同方案的土壤种子库萌发效果进行评价。研究发现:(1)相比于空白试验,正交试验的设计明显增加了土壤种子库的密度及物种丰富度,正交试验土壤种子库中萌发植物的总数为3 519株(约为空白试验的3.04倍),物种数高达39种(约为空白试验的1.22倍);(2)表土铺设厚度是3个因素中最重要的因素,而引入乔木种子的效果并不明显;(3)采用极差分析法、主成分分析法、指标体系法来评估不同方案的土壤种子库萌发效果均具有一定的合理性,但3种评价方法都存在不足,因此综合考虑3种评价方法可以提高评价效果的合理性。经过综合分析,8号恢复方案(A3B2C1,即表土铺设厚度为5 cm,覆盖物厚度为3 cm,引入种子数量为100ind·m-2)具有最佳恢复效果,可以为植被恢复提供借鉴。
[Abstract]:Soil seed bank (Soil Seed Bank) is the summation of all viable seeds in the soil and the deciduous layer of soil surface, and it is also a provenance bank for natural regeneration of vegetation. In this paper, the surface soil laying thickness, covering thickness and tree seed number are selected to set up orthogonal experiment, and the characteristics of soil seed bank after orthogonal test are analyzed, and the range analysis method and principal component analysis method are used to analyze the characteristics of soil seed bank. Index system method was used to evaluate the germinating effect of soil seed bank. The results showed that: (1) compared with the blank experiment, the design of orthogonal experiment increased the density and species richness of soil seed bank. The total number of germinating plants in the soil seed bank of orthogonal experiment was 3 519 (about 3.04 times of blank test), and 39 species (about 1.22 times); (2 of blank test) were the most important factors among the three factors. But the effect of introducing Arbor seed is not obvious. (3) it is reasonable to use range analysis, principal component analysis and index system method to evaluate the germination effect of soil seed bank of different schemes, but the three evaluation methods are all insufficient. Therefore, comprehensive consideration of three evaluation methods can improve the reasonableness of evaluation effect. According to the comprehensive analysis, the best restoration effect of the No. 8 restoration scheme (A _ 3B _ 2C _ 1, that is, the surface soil covering thickness of 5 cm, mulch thickness is 3 cm, and the seed number is 100ind m ~ (-2) can be used for reference for vegetation restoration.
【作者单位】：南开大学环境科学与工程学院;天津师范大学城市与环境科学学院;
【基金】：国家自然科学基金项目(31370700)
【分类号】：S154

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