关帝山森林土壤真菌群落结构与遗传多样性特征

发布时间：2018-09-13 11:36

【摘要】：土壤微生物群落驱动土壤碳、氮、磷、硫生物地球化学循环,在维持土壤碳汇和生态系统功能等方面发挥重要的作用.分析环境与空间因素在寒温性针叶林土壤微生物群落构建中的作用,可以为区域森林生态系统管理措施的制定提供理论依据.本文利用Illumina高通量测序技术分析了关帝山庞泉沟自然保护区华北落叶松(Larix principis-rupprechtii)林、青gD(Picea wilsonii)林、油松(Pinus tabulaeformis)林以及桦树(Betula spp.)林这4种林型的5个土壤真菌群落(Lp MC1、Lp MC2、Pw MC、Pt MC和BMC)的结构.同时测定林下土壤环境因子及林下植被多样性,分析真菌群落的结构与植被和土壤环境因子的相关性.结果表明:(1)5个样地中共有7个真菌门和33个优势真菌属;(2)冗余分析结果表明:土壤pH、温度、含水率、全氮含量、NH+4含量、全碳含量、蔗糖酶活性、脲酶活性、林下植被优势度和均匀度与土壤真菌群落结构显著相关;(3)聚类分析和PCA分析结果表明,森林植被类型、土壤环境因子和林下植被对土壤真菌群落结构影响显著.(4)空间变量(PCNM)分析结果表明,在局域尺度(local scale)上扩散限制对研究区域真菌群落构建的影响不显著.本研究区森林土壤真菌群落结构主要受到环境选择(土壤pH、温度、含水率、全氮含量、NH+4含量、全碳含量、蔗糖酶活性、脲酶活性、林下植被优势度和均匀度、森林类型)的显著影响.
[Abstract]:Soil microbial communities drive the biogeochemical cycle of soil carbon, nitrogen, phosphorus and sulfur, and play an important role in maintaining soil carbon sinks and ecosystem functions. Analyzing the role of environment and space factors in soil microbial community construction of cold-temperate coniferous forest can provide theoretical basis for the establishment of regional forest ecosystem management measures. In this paper, Illumina high throughput sequencing technique was used to analyze the Larix principis-rupprechtii forest, green gD (Picea wilsonii) forest, (Pinus tabulaeformis) forest and (Betula spp.) of birch in Pangquanggou Nature Reserve of Guandi Mountain. Structure of five soil fungal communities (Lp MC1,Lp MC2,Pw MC,Pt MC and BMC) in these four forest types. At the same time, the soil environmental factors and the diversity of undergrowth vegetation were measured, and the correlation between the structure of fungal community and vegetation and soil environmental factors was analyzed. The results were as follows: (1) there were 7 phylum fungi and 33 dominant fungi genera in 5 plots; (2) the results of redundancy analysis showed that soil pH, temperature, moisture content, total nitrogen content, NH _ 4 content, total carbon content, sucrase activity, urease activity, total carbon content, urease activity, total nitrogen content, total carbon content, urease activity, total The dominance and evenness of understory vegetation were significantly correlated with the soil fungal community structure. (3) the results of cluster analysis and PCA analysis showed that the forest vegetation types, Soil environmental factors and understory vegetation had significant effects on the structure of soil fungal community. (4) the results of spatial variable (PCNM) analysis showed that diffusion restriction on the local scale (local scale) had no significant effect on the construction of fungal communities in the study region. The community structure of forest soil fungi was mainly selected by environment (soil pH, temperature, moisture content, total nitrogen content NH _ 4 content, total carbon content, sucrase activity, urease activity, dominance and evenness of undergrowth vegetation. Forest type).
【作者单位】：山西大学黄土高原研究所;山西财经大学环境经济学院;
【基金】：山西省自然科学基金项目(201601D102054) 国家自然科学基金青年科学基金项目(31600308) 山西省回国留学人员科研资助项目(2012-059)
【分类号】：S714.3

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