土壤置换法研究土壤基质对微生物碳氮磷的影响

发布时间：2018-10-09 16:08

【摘要】：【目的】探索植被演替初期不同土壤基质对微生物碳氮磷含量及其化学计量比的影响。【方法】于2004年采集天然次生林(演替前样地)的A层(淋溶层)、B层(淀积层)和C层(母质层土)土,分别填入30 cm土层厚度的样地,构成A、B和C处理,分别模拟森林皆伐次生演替、无种子库次生演替和原生演替,每种处理4个重复,经过10 a自然演替,比较不同土壤基质间微生物碳氮磷含量及其化学计量比的差异。【结果】(1)演替前样地不同土壤基质微生物生物量碳氮磷及其占土壤碳氮磷的比例均呈现出A处理显著高于B、C两个处理(P0.05,C处理微生物生物量氮占土壤氮比例除外);土壤微生物生物量碳氮比(n_(Cmic)/n_(Nmic))为A处理低于B、C处理,土壤微生物生物量碳磷比(n_(Cmic)/n_(Pmic))和微生物生物量氮磷比(n_(Nmic)/n_(Pmic))均为A处理高于B、C处理;(2)演替后样地,不同土壤基质微生物生物量碳氮磷呈现出A处理显著高于B、C两个处理(P0.05),其占土壤碳氮磷的比例均呈现出A处理高于B、C两个处理;n_(Cmic)/n_(Nmic)为A、B处理低于C处理,n_(Cmic)/n_(Pmic)和n_(Nmic)/n_(Pmic)均为A处理高于B、C处理;(3)经过10 a植被演替,A、B、C 3个处理土壤微生物生物量碳氮磷浓度及其占土壤碳氮磷的比例均上升(B处理n_(Pmic)除外);3个处理n_(Cmic)/n_(Nmic)下降、n_(Cmic)/n_(Pmic)和n_(Nmic)/n_(Pmic)上升,其中,B、C两个处理的n_(Cmic)/n_(Nmic)显著下降(P0.05),A、C两个处理的n_(Nmic)/n_(Pmic)显著上升(P0.05)。【结论】植被演替初期,不同土壤基质理化性质的差异是影响土壤微生物碳氮磷的主要因素。
[Abstract]:[objective] to explore the effects of different soil substrates on microbial carbon, nitrogen, phosphorus and their stoichiometric ratios in the early stage of vegetation succession. [methods] A (leaching layer) layer A (leaching layer) of natural secondary forest (pre-succession plot) was collected in 2004. Accumulation layer) and C layer (parent layer soil) soil, A sample plot with a thickness of 30 cm soil layer was filled into the sample, which was used to simulate the secondary succession of forest clear cutting, no seed bank secondary succession and primary succession respectively. Each treatment was repeated 4 times, and after 10 years of natural succession, there was no secondary succession, no seed bank, no secondary succession and no primary succession. The differences of microbial carbon, nitrogen and phosphorus contents and their stoichiometric ratios among different soil substrates were compared. [results] (1) the microbial biomass carbon, nitrogen, phosphorus and their proportion to soil carbon, nitrogen and phosphorus of different soil substrates in the sample plots before succession showed A position. The soil microbial biomass nitrogen ratio (n _ (Cmic) / n _ (Nmic) was significantly higher than that of the two treatments (except the ratio of microbial biomass nitrogen to soil nitrogen) in P0.05C treatment (n _ (Cmic) / n _ (Nmic) = A was lower than that of BnC treatment). Soil microbial biomass, carbon and phosphorus ratios (n _ (Cmic) / n _ (Pmic) and N _ (Nmic) / n _ (Pmic) were higher than those in B _ C treatment (n _ (Nmic) / n _ (Pmic). Microbial biomass carbon, nitrogen and phosphorus in different soil substrates were significantly higher in A treatment than in Bu C treatment (P0.05), and the proportion of carbon nitrogen and phosphorus in soil under A treatment was significantly higher than that in Bu C treatment (P 0.05). The ratio of N _ (Cmic) / n _ (Nmic) in A treatment was significantly higher than that in C treatment (P 0.05), and the ratio of N _ (Cmic) / n _ (Pmic) and n _ (Nmic) / n _ (Pmic) in treatment A was lower than that in C treatment (P < 0.05). (3) soil microbial biomass carbon, nitrogen and phosphorus concentrations and their proportion to soil carbon, nitrogen and phosphorus increased after 10 years of vegetation succession treatment (except for treatment B, n _ (Pmic), and n _ (Cmic) / n _ (Nmic) decreased by n _ (Cmic) _ n _ (Pmic) and n _ (Nmic) _ n _ (Pmic), respectively. The n _ (Cmic) / n _ (Nmic) of the two treatments decreased significantly (P0.05), and the n _ (Nmic) / n _ (Pmic) of the two treatments increased significantly (P0.05). [conclusion] in the early stage of vegetation succession, the physical and chemical properties of different soil substrates were the main factors affecting soil microbial carbon, nitrogen and phosphorus.
【作者单位】：东北林业大学生态研究中心;
【基金】：“十二五”国家科技支撑计划(2011BAD-37B01) 教育部长江学者和创新团队发展计划(IRT-15R09)
【分类号】：S714.3

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